2g7b

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[[Image:2g7b.gif|left|200px]]<br /><applet load="2g7b" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2g7b.gif|left|200px]]
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caption="2g7b, resolution 1.180&Aring;" />
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'''Crystal Structure of the R132K:R111L:L121E mutant of Cellular Retinoic Acid Binding Protein Type II In Complex With All-Trans-Retinal At 1.18 Angstroms Resolution'''<br />
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{{Structure
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|PDB= 2g7b |SIZE=350|CAPTION= <scene name='initialview01'>2g7b</scene>, resolution 1.180&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=AZE:ALL-TRANS AXEROPHTHENE'>AZE</scene>
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|ACTIVITY=
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|GENE= CRABP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''Crystal Structure of the R132K:R111L:L121E mutant of Cellular Retinoic Acid Binding Protein Type II In Complex With All-Trans-Retinal At 1.18 Angstroms Resolution'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2G7B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=AZE:'>AZE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G7B OCA].
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2G7B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G7B OCA].
==Reference==
==Reference==
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Protein design: reengineering cellular retinoic acid binding protein II into a rhodopsin protein mimic., Vasileiou C, Vaezeslami S, Crist RM, Rabago-Smith M, Geiger JH, Borhan B, J Am Chem Soc. 2007 May 16;129(19):6140-8. Epub 2007 Apr 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17447762 17447762]
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Protein design: reengineering cellular retinoic acid binding protein II into a rhodopsin protein mimic., Vasileiou C, Vaezeslami S, Crist RM, Rabago-Smith M, Geiger JH, Borhan B, J Am Chem Soc. 2007 May 16;129(19):6140-8. Epub 2007 Apr 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17447762 17447762]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: retinal]]
[[Category: retinal]]
[[Category: retinoic acid]]
[[Category: retinoic acid]]
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[[Category: retinoids]]
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[[Category: retinoid]]
[[Category: schiff base]]
[[Category: schiff base]]
[[Category: x-ray]]
[[Category: x-ray]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:28:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:01:18 2008''

Revision as of 15:01, 20 March 2008

Image:2g7b.gif


PDB ID 2g7b

Drag the structure with the mouse to rotate
, resolution 1.180Å
Ligands: and
Gene: CRABP2 (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of the R132K:R111L:L121E mutant of Cellular Retinoic Acid Binding Protein Type II In Complex With All-Trans-Retinal At 1.18 Angstroms Resolution


Overview

Rational redesign of the binding pocket of Cellular Retinoic Acid Binding Protein II (CRABPII) has provided a mutant that can bind retinal as a protonated Schiff base, mimicking the binding observed in rhodopsin. The reengineering was accomplished through a series of choreographed manipulations to ultimately orient the reactive species (the epsilon-amino group of Lys132 and the carbonyl of retinal) in the proper geometry for imine formation. The guiding principle was to achieve the appropriate Burgi-Dunitz trajectory for the reaction to ensue. Through crystallographic analysis of protein mutants incapable of forming the requisite Schiff base, a highly ordered water molecule was identified as a key culprit in orienting retinal in a nonconstructive manner. Removal of the ordered water, along with placing reinforcing mutations to favor the desired orientation of retinal, led to a triple mutant CRABPII protein capable of nanomolar binding of retinal as a protonated Schiff base. The high-resolution crystal structure of all-trans-retinal bound to the CRABPII triple mutant (1.2 A resolution) unequivocally illustrates the imine formed between retinal and the protein.

About this Structure

2G7B is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Protein design: reengineering cellular retinoic acid binding protein II into a rhodopsin protein mimic., Vasileiou C, Vaezeslami S, Crist RM, Rabago-Smith M, Geiger JH, Borhan B, J Am Chem Soc. 2007 May 16;129(19):6140-8. Epub 2007 Apr 21. PMID:17447762

Page seeded by OCA on Thu Mar 20 17:01:18 2008

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