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4j6e
From Proteopedia
(Difference between revisions)
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| - | {{STRUCTURE_4j6e| PDB=4j6e | SCENE= }} | ||
| - | ===Structure of LPXI D225A Mutant=== | ||
| - | {{ABSTRACT_PUBMED_23042606}} | ||
| - | == | + | ==Structure of LPXI D225A Mutant== |
| - | [[4j6e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/ | + | <StructureSection load='4j6e' size='340' side='right' caption='[[4j6e]], [[Resolution|resolution]] 2.52Å' scene=''> |
| - | [[ | + | == Structural highlights == |
| + | <table><tr><td colspan='2'>[[4j6e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Caucn Caucn]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4ggi 4ggi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J6E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J6E FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UDG:(2R,3R,4R,5S,6R)-2-{[(S)-{[(S)-{[(2R,3S,4R,5R)-5-(2,4-DIOXO-3,4-DIHYDROPYRIMIDIN-1(2H)-YL)-3,4-DIHYDROXYTETRAHYDROFURAN-2-YL]METHOXY}(HYDROXY)PHOSPHORYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-5-HYDROXY-6-(HYDROXYMETHYL)-3-{[(3R)-3-HYDROXYTETRADECANOYL]AMINO}TETRAHYDRO-2H-PYRAN-4-YL+(3R)-3-HYDROXYTETRADECANOATE'>UDG</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ggm|4ggm]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">77330127, CCNA_01987, CC_1910, lpxI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=565050 CAUCN])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-2,3-diacylglucosamine_diphosphatase UDP-2,3-diacylglucosamine diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.54 3.6.1.54] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j6e OCA], [http://pdbe.org/4j6e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j6e RCSB], [http://www.ebi.ac.uk/pdbsum/4j6e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j6e ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Enzymes in lipid metabolism acquire and deliver hydrophobic substrates and products from within lipid bilayers. The structure at 2.55 A of one isozyme of a constitutive enzyme in lipid A biosynthesis, LpxI from Caulobacter crescentus, has a novel fold. Two domains close around a completely sequestered substrate, UDP-2,3-diacylglucosamine, and open to release products either to the neighboring enzyme in a putative multienzyme complex or to the bilayer. Mutation analysis identifies Asp225 as key to Mg(2+)-catalyzed diphosphate hydrolysis. These structures provide snapshots of the enzymatic synthesis of a critical lipid A precursor. | ||
| + | |||
| + | LpxI structures reveal how a lipid A precursor is synthesized.,Metzger LE 4th, Lee JK, Finer-Moore JS, Raetz CR, Stroud RM Nat Struct Mol Biol. 2012 Nov;19(11):1132-8. doi: 10.1038/nsmb.2393. Epub 2012, Oct 7. PMID:23042606<ref>PMID:23042606</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 4j6e" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Caucn]] | ||
[[Category: UDP-2,3-diacylglucosamine diphosphatase]] | [[Category: UDP-2,3-diacylglucosamine diphosphatase]] | ||
| - | [[Category: CSMP, Center for Structures of Membrane Proteins | + | [[Category: CSMP, Center for Structures of Membrane Proteins]] |
| - | [[Category: Finer-Moore, J S | + | [[Category: Finer-Moore, J S]] |
| - | [[Category: IV, L E.Metzger | + | [[Category: IV, L E.Metzger]] |
| - | [[Category: Lee, J K | + | [[Category: Lee, J K]] |
| - | [[Category: Raetz, C R.H | + | [[Category: Raetz, C R.H]] |
| - | [[Category: Stroud, R M | + | [[Category: Stroud, R M]] |
[[Category: Center for structures of membrane protein]] | [[Category: Center for structures of membrane protein]] | ||
[[Category: Csmp]] | [[Category: Csmp]] | ||
Revision as of 08:42, 4 August 2016
Structure of LPXI D225A Mutant
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Categories: Caucn | UDP-2,3-diacylglucosamine diphosphatase | CSMP, Center for Structures of Membrane Proteins | Finer-Moore, J S | IV, L E.Metzger | Lee, J K | Raetz, C R.H | Stroud, R M | Center for structures of membrane protein | Csmp | Hydrolase | Lipid binding | Psi-biology | Structural genomic
