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3pkm

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{{STRUCTURE_3pkm| PDB=3pkm | SCENE= }}
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==Crystal structure of Cas6 with its substrate RNA==
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===Crystal structure of Cas6 with its substrate RNA===
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<StructureSection load='3pkm' size='340' side='right' caption='[[3pkm]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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{{ABSTRACT_PUBMED_21300293}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3pkm]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PKM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PKM FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF1131 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 Pyrococcus furiosus])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pkm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pkm RCSB], [http://www.ebi.ac.uk/pdbsum/3pkm PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats) found in prokaryotic genomes confer small RNA-mediated protection against viruses and other invaders. CRISPR loci contain iterations of a short repeat sequence alternating with small segments of varying invader-derived sequences. Distinct families of CRISPR-associated Cas proteins function to cleave within the repeat sequence of CRISPR transcripts and produce the individual invader-targeting crRNAs. Here, we report the crystal structure of Pyrococcus furiosus Cas6 bound with a repeat RNA at 3.2 A resolution. In contrast to other Cas families of endonucleases, Cas6 clasps nucleotides 2-9 of the repeat RNA using its two ferredoxin-like domains, and the enzyme-anchored 5' end tethers the distal cleavage site of the RNA between nucleotides 22 and 23 to the predicted enzyme active site on the opposite side of the ferrodoxin-like domains. Our findings suggest a wrap-around mechanism for CRISPR RNA recognition and cleavage by Cas6 and related processing endonucleases.
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==Function==
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Interaction of the Cas6 Riboendonuclease with CRISPR RNAs: Recognition and Cleavage.,Wang R, Preamplume G, Terns MP, Terns RM, Li H Structure. 2011 Feb 9;19(2):257-64. PMID:21300293<ref>PMID:21300293</ref>
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[[http://www.uniprot.org/uniprot/Q8U1S4_PYRFU Q8U1S4_PYRFU]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), also called psiRNA (prokaryotic silencing) in this organism. Processes pre-crRNA into individual crRNA units, with an 8-nt 5'-repeat DNA tag that may help other proteins recognize the crRNA. Further processing occurs at their 3' termini in this organism. Generates a 5' hydroxy and 2',3'-cyclic phosphodiester.<ref>PMID:20884784</ref> <ref>PMID:19141480</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3pkm]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PKM OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:021300293</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
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[[Category: Li, H.]]
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[[Category: Li, H]]
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[[Category: Preamplume, G.]]
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[[Category: Preamplume, G]]
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[[Category: Wang, R.]]
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[[Category: Wang, R]]
[[Category: Cas6]]
[[Category: Cas6]]
[[Category: Crispr]]
[[Category: Crispr]]

Revision as of 10:40, 19 December 2014

Crystal structure of Cas6 with its substrate RNA

3pkm, resolution 3.10Å

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