2gdc
From Proteopedia
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| - | [[Image:2gdc.gif|left|200px]] | + | [[Image:2gdc.gif|left|200px]] |
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| - | '''Structure of Vinculin VD1 / IpaA560-633 complex''' | + | {{Structure |
| + | |PDB= 2gdc |SIZE=350|CAPTION= <scene name='initialview01'>2gdc</scene>, resolution 2.74Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= VCL, VINC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 Gallus gallus]), ipaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 Shigella flexneri]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of Vinculin VD1 / IpaA560-633 complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2GDC is a [ | + | 2GDC is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GDC OCA]. |
==Reference== | ==Reference== | ||
| - | Structural mimicry for vinculin activation by IpaA, a virulence factor of Shigella flexneri., Hamiaux C, van Eerde A, Parsot C, Broos J, Dijkstra BW, EMBO Rep. 2006 Aug;7(8):794-9. Epub 2006 Jul 7. PMID:[http:// | + | Structural mimicry for vinculin activation by IpaA, a virulence factor of Shigella flexneri., Hamiaux C, van Eerde A, Parsot C, Broos J, Dijkstra BW, EMBO Rep. 2006 Aug;7(8):794-9. Epub 2006 Jul 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16826238 16826238] |
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: shigella flexneri]] | [[Category: shigella flexneri]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:03:19 2008'' |
Revision as of 15:03, 20 March 2008
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| , resolution 2.74Å | |||||||
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| Gene: | VCL, VINC1 (Gallus gallus), ipaA (Shigella flexneri) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of Vinculin VD1 / IpaA560-633 complex
Overview
Invasion of epithelial cells by Shigella flexneri is characterized by cytoskeletal rearrangements of the host cell membrane, promoting internalization of the bacterium. The bacterial effector IpaA is injected into the epithelial cell by a type III secretion apparatus and recruits vinculin to regulate actin polymerization at the site of entry. We analysed the complex formed between a carboxy-terminal fragment of IpaA (IpaA(560-633)) and the vinculin D1 domain (VD1), both in crystals and in solution. We present evidence that IpaA(560-633) has two alpha-helical vinculin-binding sites that simultaneously bind two VD1 molecules. The interaction of IpaA(560-633) with VD1 is highly similar to the interaction of the endogenous, eukaryotic proteins talin and alpha-actinin with VD1, showing that Shigella uses a structural mimicry strategy to activate vinculin.
About this Structure
2GDC is a Protein complex structure of sequences from Gallus gallus and Shigella flexneri. Full crystallographic information is available from OCA.
Reference
Structural mimicry for vinculin activation by IpaA, a virulence factor of Shigella flexneri., Hamiaux C, van Eerde A, Parsot C, Broos J, Dijkstra BW, EMBO Rep. 2006 Aug;7(8):794-9. Epub 2006 Jul 7. PMID:16826238
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