3qmb
From Proteopedia
(Difference between revisions)
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| - | + | ==Structural Basis of Selective Binding of Nonmethylated CpG Islands by the CXXC Domain of CFP1== | |
| - | + | <StructureSection load='3qmb' size='340' side='right' caption='[[3qmb]], [[Resolution|resolution]] 2.06Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[3qmb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QMB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QMB FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qmc|3qmc]], [[3qmd|3qmd]], [[3qmg|3qmg]], [[3qmh|3qmh]], [[3qmi|3qmi]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CFP1, CGBP, CXXC1, PCCX1, PHF18 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qmb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qmb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qmb RCSB], [http://www.ebi.ac.uk/pdbsum/3qmb PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/CXXC1_HUMAN CXXC1_HUMAN]] Transcriptional activator that exhibits a unique DNA binding specificity for CpG unmethylated motifs with a preference for CpGG.<ref>PMID:21407193</ref> | [[http://www.uniprot.org/uniprot/CXXC1_HUMAN CXXC1_HUMAN]] Transcriptional activator that exhibits a unique DNA binding specificity for CpG unmethylated motifs with a preference for CpGG.<ref>PMID:21407193</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | CFP1 is a CXXC domain-containing protein and an essential component of the SETD1 histone H3K4 methyltransferase complex. CXXC domain proteins direct different chromatin-modifying activities to various chromatin regions. Here, we report crystal structures of the CFP1 CXXC domain in complex with six different CpG DNA sequences. The crescent-shaped CFP1 CXXC domain is wedged into the major groove of the CpG DNA, distorting the B-form DNA, and interacts extensively with the major groove of the DNA. The structures elucidate the molecular mechanism of the non-methylated CpG-binding specificity of the CFP1 CXXC domain. The CpG motif is confined by a tripeptide located in a rigid loop, which only allows the accommodation of the non-methylated CpG dinucleotide. Furthermore, we demonstrate that CFP1 has a preference for a guanosine nucleotide following the CpG motif. | ||
| - | + | The structural basis for selective binding of non-methylated CpG islands by the CFP1 CXXC domain.,Xu C, Bian C, Lam R, Dong A, Min J Nat Commun. 2011 Mar;2:227. PMID:21407193<ref>PMID:21407193</ref> | |
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| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Arrowsmith, C H | + | [[Category: Arrowsmith, C H]] |
| - | [[Category: Bian, C B | + | [[Category: Bian, C B]] |
| - | [[Category: Bochkarev, A | + | [[Category: Bochkarev, A]] |
| - | [[Category: Bountra, C | + | [[Category: Bountra, C]] |
| - | [[Category: Edwards, A M | + | [[Category: Edwards, A M]] |
| - | [[Category: Kania, J | + | [[Category: Kania, J]] |
| - | [[Category: Lam, R | + | [[Category: Lam, R]] |
| - | [[Category: Min, J | + | [[Category: Min, J]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: Weigelt, J | + | [[Category: Weigelt, J]] |
| - | [[Category: Xu, C | + | [[Category: Xu, C]] |
[[Category: Cxxc-type zn finger]] | [[Category: Cxxc-type zn finger]] | ||
[[Category: Dna binding]] | [[Category: Dna binding]] | ||
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[[Category: Nucleus speckle]] | [[Category: Nucleus speckle]] | ||
[[Category: Sgc]] | [[Category: Sgc]] | ||
| - | [[Category: Structural genomics consortium]] | ||
[[Category: Unmethylated cpg motif]] | [[Category: Unmethylated cpg motif]] | ||
Revision as of 08:23, 24 December 2014
Structural Basis of Selective Binding of Nonmethylated CpG Islands by the CXXC Domain of CFP1
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