2ger
From Proteopedia
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| - | [[Image:2ger.gif|left|200px]] | + | [[Image:2ger.gif|left|200px]] |
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| - | '''Crystal Structure and Oxidative Mechanism of Human Pyrroline-5-carboxylate Reductase''' | + | {{Structure |
| + | |PDB= 2ger |SIZE=350|CAPTION= <scene name='initialview01'>2ger</scene>, resolution 3.1Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Pyrroline-5-carboxylate_reductase Pyrroline-5-carboxylate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.2 1.5.1.2] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure and Oxidative Mechanism of Human Pyrroline-5-carboxylate Reductase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2GER is a [ | + | 2GER is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GER OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of human pyrroline-5-carboxylate reductase., Meng Z, Lou Z, Liu Z, Li M, Zhao X, Bartlam M, Rao Z, J Mol Biol. 2006 Jun 23;359(5):1364-77. Epub 2006 May 11. PMID:[http:// | + | Crystal structure of human pyrroline-5-carboxylate reductase., Meng Z, Lou Z, Liu Z, Li M, Zhao X, Bartlam M, Rao Z, J Mol Biol. 2006 Jun 23;359(5):1364-77. Epub 2006 May 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16730026 16730026] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Pyrroline-5-carboxylate reductase]] | [[Category: Pyrroline-5-carboxylate reductase]] | ||
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[[Category: oxidative mechanism of human pyrroline-5-carboxylate]] | [[Category: oxidative mechanism of human pyrroline-5-carboxylate]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:03:52 2008'' |
Revision as of 15:03, 20 March 2008
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| , resolution 3.1Å | |||||||
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| Activity: | Pyrroline-5-carboxylate reductase, with EC number 1.5.1.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure and Oxidative Mechanism of Human Pyrroline-5-carboxylate Reductase
Overview
Pyrroline-5-carboxylate reductase (P5CR) is a universal housekeeping enzyme that catalyzes the reduction of Delta(1)-pyrroline-5-carboxylate (P5C) to proline using NAD(P)H as the cofactor. The enzymatic cycle between P5C and proline is very important for the regulation of amino acid metabolism, intracellular redox potential, and apoptosis. Here, we present the 2.8 Angstroms resolution structure of the P5CR apo enzyme, its 3.1 Angstroms resolution ternary complex with NAD(P)H and substrate-analog. The refined structures demonstrate a decameric architecture with five homodimer subunits and ten catalytic sites arranged around a peripheral circular groove. Mutagenesis and kinetic studies reveal the pivotal roles of the dinucleotide-binding Rossmann motif and residue Glu221 in the human enzyme. Human P5CR is thermostable and the crystals were grown at 37 degrees C. The enzyme is implicated in oxidation of the anti-tumor drug thioproline.
About this Structure
2GER is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of human pyrroline-5-carboxylate reductase., Meng Z, Lou Z, Liu Z, Li M, Zhao X, Bartlam M, Rao Z, J Mol Biol. 2006 Jun 23;359(5):1364-77. Epub 2006 May 11. PMID:16730026
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