2ghy
From Proteopedia
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| - | [[Image:2ghy.gif|left|200px]] | + | [[Image:2ghy.gif|left|200px]] |
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| - | '''Novel Crystal Form of the ColE1 Rom Protein''' | + | {{Structure |
| + | |PDB= 2ghy |SIZE=350|CAPTION= <scene name='initialview01'>2ghy</scene>, resolution 2.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= rop ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Novel Crystal Form of the ColE1 Rom Protein''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2GHY is a [ | + | 2GHY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHY OCA]. |
==Reference== | ==Reference== | ||
| - | Novel crystal form of the ColE1 Rom protein., Jang SB, Jeong MS, Carter RJ, Holbrook EL, Comolli LR, Holbrook SR, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):619-27. Epub 2006, May 12. PMID:[http:// | + | Novel crystal form of the ColE1 Rom protein., Jang SB, Jeong MS, Carter RJ, Holbrook EL, Comolli LR, Holbrook SR, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):619-27. Epub 2006, May 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16699189 16699189] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Jeong, M S.]] | [[Category: Jeong, M S.]] | ||
[[Category: hiv-1]] | [[Category: hiv-1]] | ||
| - | [[Category: kissing | + | [[Category: kissing hairpin]] |
[[Category: rna one modulator protein]] | [[Category: rna one modulator protein]] | ||
[[Category: structural packing]] | [[Category: structural packing]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:04:53 2008'' |
Revision as of 15:04, 20 March 2008
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| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | rop (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Novel Crystal Form of the ColE1 Rom Protein
Overview
The RNA I modulator protein (Rom) acts as a co-regulator of ColE1 plasmid copy number by binding to RNA kissing hairpins and stabilizing their interaction. The structure of Rom has been determined in a new crystal form from X-ray diffraction data to 2.5 A resolution. In this structure, a dimer of the 57-amino-acid protein is found in the asymmetric unit. Each subunit consists almost entirely of two antiparallel alpha-helices joined by a short hairpin bend. The dimer contains a non-crystallographic twofold axis and forms a highly regular four-alpha-helical bundle. The structural packing in this novel crystal form is different from previously known Rom structures. The asymmetric unit contains one dimer, giving a crystal volume per protein weight (V(M)) of 1.83 A(3) Da(-1) and a low solvent content of 30%. Strong packing interactions and low solvation are characteristic of the structure. The Rom protein was cocrystallized with the Tar-Tar* kissing hairpin RNA. Although the electron-density maps do not show bound RNA, altered conformations in the side chains of Rom that are known to be involved in RNA binding have been identified. These results provide additional information about Rom protein conformational flexibility and suggest that the presence of a highly charged polymer such as RNA can promote tight packing of an RNA-binding protein, even when the RNA itself is not observed in the crystal.
About this Structure
2GHY is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Novel crystal form of the ColE1 Rom protein., Jang SB, Jeong MS, Carter RJ, Holbrook EL, Comolli LR, Holbrook SR, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):619-27. Epub 2006, May 12. PMID:16699189
Page seeded by OCA on Thu Mar 20 17:04:53 2008
