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2gja

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[[Image:2gja.gif|left|200px]]<br /><applet load="2gja" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2gja.gif|left|200px]]
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caption="2gja, resolution 1.85&Aring;" />
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'''Structure of the MnmE G-domain in complex with GDP*AlF4-, Mg2+ and NH4+'''<br />
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{{Structure
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|PDB= 2gja |SIZE=350|CAPTION= <scene name='initialview01'>2gja</scene>, resolution 1.85&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene> and <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene>
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|ACTIVITY=
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|GENE= trmE, mnmE, thdF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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}}
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'''Structure of the MnmE G-domain in complex with GDP*AlF4-, Mg2+ and NH4+'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2GJA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ALF:'>ALF</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NH4:'>NH4</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJA OCA].
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2GJA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJA OCA].
==Reference==
==Reference==
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Dimerisation-dependent GTPase reaction of MnmE: how potassium acts as GTPase-activating element., Scrima A, Wittinghofer A, EMBO J. 2006 Jun 21;25(12):2940-51. Epub 2006 Jun 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16763562 16763562]
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Dimerisation-dependent GTPase reaction of MnmE: how potassium acts as GTPase-activating element., Scrima A, Wittinghofer A, EMBO J. 2006 Jun 21;25(12):2940-51. Epub 2006 Jun 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16763562 16763562]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: g-domain dimer]]
[[Category: g-domain dimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:32:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:05:21 2008''

Revision as of 15:05, 20 March 2008

Image:2gja.gif


PDB ID 2gja

Drag the structure with the mouse to rotate
, resolution 1.85Å
Ligands: , , and
Gene: trmE, mnmE, thdF (Escherichia coli)
Coordinates: save as pdb, mmCIF, xml



Structure of the MnmE G-domain in complex with GDP*AlF4-, Mg2+ and NH4+


Overview

MnmE, a Guanine nucleotide-binding protein conserved between bacteria and man, is involved in the modification of tRNAs. Here we provide biochemical and X-ray structural evidence for a new GTP-hydrolysis mechanism, where the G-domains of MnmE dimerise in a potassium-dependent manner and induce GTP hydrolysis. The structure in the presence of GDP-AlFx and potassium shows how juxtaposition of the subunits induces a conformational change around the nucleotide which reorients the catalytic machinery. A critical glutamate is positioned such as to stabilise or activate the attacking water. Potassium provides a positive charge into the catalytic site in a position analogous to the arginine finger in the Ras-RasGAP system. Mutational studies show that potassium-dependent dimerisation and GTP hydrolysis can be uncoupled and that interaction between the G-domains is a prerequisite for subsequent phosphoryl transfer. We propose a model for the juxtaposition of G-domains in the full-length protein and how it induces conformational changes in the putative tRNA-modification centre.

About this Structure

2GJA is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Dimerisation-dependent GTPase reaction of MnmE: how potassium acts as GTPase-activating element., Scrima A, Wittinghofer A, EMBO J. 2006 Jun 21;25(12):2940-51. Epub 2006 Jun 8. PMID:16763562

Page seeded by OCA on Thu Mar 20 17:05:21 2008

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