2gms

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[[Image:2gms.gif|left|200px]]<br /><applet load="2gms" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2gms.gif|left|200px]]
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caption="2gms, resolution 1.8&Aring;" />
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'''E coli GDP-4-keto-6-deoxy-D-mannose-3-dehydratase with bound hydrated PLP'''<br />
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{{Structure
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|PDB= 2gms |SIZE=350|CAPTION= <scene name='initialview01'>2gms</scene>, resolution 1.8&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=P0P:[4-(DIHYDROXYMETHYL)-5-HYDROXY-6-METHYLPYRIDIN-3-YL]METHYL DIHYDROGEN PHOSPHATE'>P0P</scene>
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|ACTIVITY=
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|GENE= wbdK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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}}
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'''E coli GDP-4-keto-6-deoxy-D-mannose-3-dehydratase with bound hydrated PLP'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2GMS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=P0P:'>P0P</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GMS OCA].
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2GMS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GMS OCA].
==Reference==
==Reference==
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The structure of GDP-4-keto-6-deoxy-D-mannose-3-dehydratase: a unique coenzyme B6-dependent enzyme., Cook PD, Thoden JB, Holden HM, Protein Sci. 2006 Sep;15(9):2093-106. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16943443 16943443]
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The structure of GDP-4-keto-6-deoxy-D-mannose-3-dehydratase: a unique coenzyme B6-dependent enzyme., Cook PD, Thoden JB, Holden HM, Protein Sci. 2006 Sep;15(9):2093-106. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16943443 16943443]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: plp]]
[[Category: plp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:33:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:06:36 2008''

Revision as of 15:06, 20 March 2008

Image:2gms.gif


PDB ID 2gms

Drag the structure with the mouse to rotate
, resolution 1.8Å
Ligands: and
Gene: wbdK (Escherichia coli)
Coordinates: save as pdb, mmCIF, xml



E coli GDP-4-keto-6-deoxy-D-mannose-3-dehydratase with bound hydrated PLP


Overview

L-colitose is a 3,6-dideoxysugar found in the O-antigens of some Gram-negative bacteria such as Escherichia coli and in marine bacteria such as Pseudoalteromonas tetraodonis. The focus of this investigation, GDP-4-keto-6-deoxy-D-mannose-3-dehydratase, catalyzes the third step in colitose production, which is the removal of the hydroxyl group at C3' of GDP-4-keto-6-deoxymannose. It is an especially intriguing PLP-dependent enzyme in that it acts as both a transaminase and a dehydratase. Here we present the first X-ray structure of this enzyme isolated from E. coli Strain 5a, type O55:H7. The two subunits of the protein form a tight dimer with a buried surface area of approximately 5000 A2. This is a characteristic feature of the aspartate aminotransferase superfamily. Although the PLP-binding pocket is formed primarily by one subunit, there is a loop, delineated by Phe 240 to Glu 253 in the second subunit, that completes the active site architecture. The hydrated form of PLP was observed in one of the enzyme/cofactor complexes described here. Amino acid residues involved in anchoring the cofactor to the protein include Gly 56, Ser 57, Asp 159, Glu 162, and Ser 183 from one subunit and Asn 248 from the second monomer. In the second enzyme/cofactor complex reported, a glutamate ketimine intermediate was found trapped in the active site. Taken together, these two structures, along with previously reported biochemical data, support the role of His 188 as the active site base required for catalysis.

About this Structure

2GMS is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The structure of GDP-4-keto-6-deoxy-D-mannose-3-dehydratase: a unique coenzyme B6-dependent enzyme., Cook PD, Thoden JB, Holden HM, Protein Sci. 2006 Sep;15(9):2093-106. PMID:16943443

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