2gqq
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2gqq.jpg|left|200px]] | + | [[Image:2gqq.jpg|left|200px]] |
| - | + | ||
| - | '''Crystal Structure of E. coli Leucine-responsive regulatory protein (Lrp)''' | + | {{Structure |
| + | |PDB= 2gqq |SIZE=350|CAPTION= <scene name='initialview01'>2gqq</scene>, resolution 3.2Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= lrp, alsB, ihb, livR, oppI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of E. coli Leucine-responsive regulatory protein (Lrp)''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2GQQ is a [ | + | 2GQQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GQQ OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the Escherichia coli leucine-responsive regulatory protein Lrp reveals a novel octameric assembly., de los Rios S, Perona JJ, J Mol Biol. 2007 Mar 9;366(5):1589-602. Epub 2006 Dec 19. PMID:[http:// | + | Structure of the Escherichia coli leucine-responsive regulatory protein Lrp reveals a novel octameric assembly., de los Rios S, Perona JJ, J Mol Biol. 2007 Mar 9;366(5):1589-602. Epub 2006 Dec 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17223133 17223133] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 17: | Line 26: | ||
[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:07:52 2008'' |
Revision as of 15:07, 20 March 2008
| |||||||
| , resolution 3.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | lrp, alsB, ihb, livR, oppI (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of E. coli Leucine-responsive regulatory protein (Lrp)
Overview
The structure of Escherichia coli leucine-responsive regulatory protein (Lrp) co-crystallized with a short duplex oligodeoxynucleotide reveals a novel quaternary assembly in which the protein octamer forms an open, linear array of four dimers. In contrast, structures of the Lrp homologs LrpA, LrpC and AsnC crystallized in the absence of DNA show that these proteins instead form highly symmetrical octamers in which the four dimers form a closed ring. Although the DNA is disordered within the Lrp crystal, comparative analyses suggest that the observed differences in quaternary state may arise from DNA interactions during crystallization. Interconversion of these conformations, possibly in response to DNA or leucine binding, provides an underlying mechanism to alter the relative spatial orientation of the DNA-binding domains. Breaking of the closed octamer symmetry may be a common essential step in the formation of active DNA complexes by all members of the Lrp/AsnC family of transcriptional regulatory proteins.
About this Structure
2GQQ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure of the Escherichia coli leucine-responsive regulatory protein Lrp reveals a novel octameric assembly., de los Rios S, Perona JJ, J Mol Biol. 2007 Mar 9;366(5):1589-602. Epub 2006 Dec 19. PMID:17223133
Page seeded by OCA on Thu Mar 20 17:07:52 2008
