This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
4bh6
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==Insights into degron recognition by APC coactivators from the structure of an Acm1-Cdh1 complex== | |
| - | + | <StructureSection load='4bh6' size='340' side='right' caption='[[4bh6]], [[Resolution|resolution]] 2.90Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[4bh6]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BH6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BH6 FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bh6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bh6 RCSB], [http://www.ebi.ac.uk/pdbsum/4bh6 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/CDH1_YEAST CDH1_YEAST]] Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). During telophase and in the subsequent G1 phase of the cell cycle, recognizes and binds proteins containing a destruction box (D-box) and an additional degradation signal termed the KEN box including ASE1, CDC20, the B-type cyclins CLB2 and CLB3, the polo-like kinase CDC5 and HSL1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for exit from mitosis, cytokinesis and formation of prereplicative complexes in G1. Probably is the target of a BUB2-dependent spindle checkpoint pathway.<ref>PMID:10329618</ref> <ref>PMID:11566880</ref> <ref>PMID:11448992</ref> [[http://www.uniprot.org/uniprot/ACM1_YEAST ACM1_YEAST]] Negative regulator of GDH1, the activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C), and which is required for exit from mitosis, cytokinesis and formation of prereplicative complexes in G1.<ref>PMID:17030612</ref> | [[http://www.uniprot.org/uniprot/CDH1_YEAST CDH1_YEAST]] Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). During telophase and in the subsequent G1 phase of the cell cycle, recognizes and binds proteins containing a destruction box (D-box) and an additional degradation signal termed the KEN box including ASE1, CDC20, the B-type cyclins CLB2 and CLB3, the polo-like kinase CDC5 and HSL1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for exit from mitosis, cytokinesis and formation of prereplicative complexes in G1. Probably is the target of a BUB2-dependent spindle checkpoint pathway.<ref>PMID:10329618</ref> <ref>PMID:11566880</ref> <ref>PMID:11448992</ref> [[http://www.uniprot.org/uniprot/ACM1_YEAST ACM1_YEAST]] Negative regulator of GDH1, the activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C), and which is required for exit from mitosis, cytokinesis and formation of prereplicative complexes in G1.<ref>PMID:17030612</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The anaphase-promoting complex/cyclosome (APC/C) regulates sister chromatid segregation and the exit from mitosis. Selection of most APC/C substrates is controlled by coactivator subunits (either Cdc20 or Cdh1) that interact with substrate destruction motifs-predominantly the destruction (D) box and KEN box degrons. How coactivators recognize D box degrons and how this is inhibited by APC/C regulatory proteins is not defined at the atomic level. Here, from the crystal structure of S. cerevisiae Cdh1 in complex with its specific inhibitor Acm1, which incorporates D and KEN box pseudosubstrate motifs, we describe the molecular basis for D box recognition. Additional interactions between Acm1 and Cdh1 identify a third protein-binding site on Cdh1 that is likely to confer coactivator-specific protein functions including substrate association. We provide a structural rationalization for D box and KEN box recognition by coactivators and demonstrate that many noncanonical APC/C degrons bind APC/C coactivators at the D box coreceptor. | ||
| - | + | Insights into Degron Recognition by APC/C Coactivators from the Structure of an Acm1-Cdh1 Complex.,He J, Chao WC, Zhang Z, Yang J, Cronin N, Barford D Mol Cell. 2013 May 22. pii: S1097-2765(13)00329-8. doi:, 10.1016/j.molcel.2013.04.024. PMID:23707760<ref>PMID:23707760</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Barford, D | + | [[Category: Barford, D]] |
| - | [[Category: Chao, W C.H | + | [[Category: Chao, W C.H]] |
| - | [[Category: Cronin, N | + | [[Category: Cronin, N]] |
| - | [[Category: He, J | + | [[Category: He, J]] |
| - | [[Category: Yang, J | + | [[Category: Yang, J]] |
| - | [[Category: Zhang, Z | + | [[Category: Zhang, Z]] |
[[Category: Acm1]] | [[Category: Acm1]] | ||
[[Category: Anaphase promoting complex]] | [[Category: Anaphase promoting complex]] | ||
Revision as of 20:45, 25 December 2014
Insights into degron recognition by APC coactivators from the structure of an Acm1-Cdh1 complex
| |||||||||||
