2hb5
From Proteopedia
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| - | [[Image:2hb5.gif|left|200px]] | + | [[Image:2hb5.gif|left|200px]] |
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| - | '''Crystal Structure of the Moloney Murine Leukemia Virus RNase H Domain''' | + | {{Structure |
| + | |PDB= 2hb5 |SIZE=350|CAPTION= <scene name='initialview01'>2hb5</scene>, resolution 1.59Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= POL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11801 Moloney murine leukemia virus]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the Moloney Murine Leukemia Virus RNase H Domain''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HB5 is a [ | + | 2HB5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HB5 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the moloney murine leukemia virus RNase H domain., Lim D, Gregorio GG, Bingman C, Martinez-Hackert E, Hendrickson WA, Goff SP, J Virol. 2006 Sep;80(17):8379-89. PMID:[http:// | + | Crystal structure of the moloney murine leukemia virus RNase H domain., Lim D, Gregorio GG, Bingman C, Martinez-Hackert E, Hendrickson WA, Goff SP, J Virol. 2006 Sep;80(17):8379-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16912289 16912289] |
[[Category: Moloney murine leukemia virus]] | [[Category: Moloney murine leukemia virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: rnase h]] | [[Category: rnase h]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:14:52 2008'' |
Revision as of 15:14, 20 March 2008
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| , resolution 1.59Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | POL (Moloney murine leukemia virus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Moloney Murine Leukemia Virus RNase H Domain
Overview
A crystallographic study of the Moloney murine leukemia virus (Mo-MLV) RNase H domain was performed to provide information about its structure and mechanism of action. These efforts resulted in the crystallization of a mutant Mo-MLV RNase H lacking the putative helix C (DeltaC). The 1.6-Angstroms resolution structure resembles the known structures of the human immunodeficiency virus type 1 (HIV-1) and Escherichia coli RNase H. The structure revealed the coordination of a magnesium ion within the catalytic core comprised of the highly conserved acidic residues D524, E562, and D583. Surface charge mapping of the Mo-MLV structure revealed a high density of basic charges on one side of the enzyme. Using a model of the Mo-MLV structure superimposed upon a structure of HIV-1 reverse transcriptase bound to an RNA/DNA hybrid substrate, Mo-MLV RNase H secondary structures and individual amino acids were examined for their potential roles in binding substrate. Identified regions included Mo-MLV RNase H beta1-beta2, alphaA, and alphaB and residues from alphaB to alphaD and its following loop. Most of the identified substrate-binding residues corresponded with residues directly binding nucleotides in an RNase H from Bacillus halodurans as observed in a cocrystal structure with RNA/DNA. Finally, superimposition of RNases H of Mo-MLV, E. coli, and HIV-1 revealed that a loop of the HIV-1 connection domain resides within the same region of the Mo-MLV and E. coli C-helix. The HIV-1 connection domain may serve to recognize and bind the RNA/DNA substrate major groove.
About this Structure
2HB5 is a Single protein structure of sequence from Moloney murine leukemia virus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the moloney murine leukemia virus RNase H domain., Lim D, Gregorio GG, Bingman C, Martinez-Hackert E, Hendrickson WA, Goff SP, J Virol. 2006 Sep;80(17):8379-89. PMID:16912289
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