3vm7
From Proteopedia
(Difference between revisions)
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| - | + | ==Structure of an Alpha-Amylase from Malbranchea cinnamomea== | |
| - | + | <StructureSection load='3vm7' size='340' side='right' caption='[[3vm7]], [[Resolution|resolution]] 2.25Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3vm7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Malbranchea_cinnamomea Malbranchea cinnamomea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VM7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VM7 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vm7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vm7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vm7 RCSB], [http://www.ebi.ac.uk/pdbsum/3vm7 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A novel alpha-amylase (McAmyA) from the thermophilic fungus, Malbranchea cinnamomea was purified, characterized and crystallized in the present study. McAmyA was purified to apparent homogeneity with a molecular mass of 60.3 kDa on SDS-PAGE. The enzyme exhibited maximal activity at pH 6.5 and was stable within pH 5.0-10.0. It was most active at 65 degrees C and was stable up to 50 degrees C. McAmyA was capable of hydrolyzing amylose, starch, amylopectin, pullulan, cyclodextrins and maltooligosaccharides. The full-length cDNA of an alpha-amylase gene (McAmyA) from the strain was cloned. McAmyA consisted of a 1,476-bp open reading frame encoding 492 amino acids. It displayed the highest amino acid sequence homology (less than 60 %) with the reported alpha-amylases. The crystal structure of McAmyA was solved at a resolution of 2.25 A (PDB code 3VM7). The overall structure of McAmyA reveals three domains with ten alpha helices and 14 beta strands, and the putative catalytic residues are positioned at domain A with somewhat different secondary structural circumstances compared with typical alpha-amylases. | ||
| - | + | A novel multifunctional alpha-amylase from the thermophilic fungus Malbranchea cinnamomea: biochemical characterization and three-dimensional structure.,Han P, Zhou P, Hu S, Yang S, Yan Q, Jiang Z Appl Biochem Biotechnol. 2013 May;170(2):420-35. doi: 10.1007/s12010-013-0198-y. , Epub 2013 Mar 29. PMID:23536251<ref>PMID:23536251</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | |||
| + | ==See Also== | ||
| + | *[[Amylase|Amylase]] | ||
| + | *[[User:Gabriel Pons/Sandbox 2|User:Gabriel Pons/Sandbox 2]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Malbranchea cinnamomea]] | [[Category: Malbranchea cinnamomea]] | ||
| - | [[Category: Han, P | + | [[Category: Han, P]] |
| - | [[Category: Hu, S Q | + | [[Category: Hu, S Q]] |
| - | [[Category: Jiang, Z Q | + | [[Category: Jiang, Z Q]] |
| - | [[Category: Yang, S Q | + | [[Category: Yang, S Q]] |
| - | [[Category: Zhou, P | + | [[Category: Zhou, P]] |
| - | [[Category: Zhou, Y | + | [[Category: Zhou, Y]] |
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Malbranchea cinnamomea c -amylase cmodel]] | [[Category: Malbranchea cinnamomea c -amylase cmodel]] | ||
[[Category: Protein conformation]] | [[Category: Protein conformation]] | ||
Revision as of 10:26, 4 January 2015
Structure of an Alpha-Amylase from Malbranchea cinnamomea
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