2hcy
From Proteopedia
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| - | [[Image:2hcy.gif|left|200px]] | + | [[Image:2hcy.gif|left|200px]] |
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| - | '''Yeast Alcohol Dehydrogenase I, Saccharomyces cerevisiae fermentative enzyme''' | + | {{Structure |
| + | |PDB= 2hcy |SIZE=350|CAPTION= <scene name='initialview01'>2hcy</scene>, resolution 2.44Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=8ID:NICOTINAMIDE-8-IODO-ADENINE-DINUCLEOTIDE'>8ID</scene> and <scene name='pdbligand=ETF:TRIFLUOROETHANOL'>ETF</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1] | ||
| + | |GENE= ADH1, ADC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''Yeast Alcohol Dehydrogenase I, Saccharomyces cerevisiae fermentative enzyme''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HCY is a [ | + | 2HCY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HCY OCA]. |
==Reference== | ==Reference== | ||
| - | Crystallization and preliminary crystallographic studies of Saccharomyces cerevisiae alcohol dehydrogenase I., Ramaswamy S, Kratzer DA, Hershey AD, Rogers PH, Arnone A, Eklund H, Plapp BV, J Mol Biol. 1994 Jan 14;235(2):777-9. PMID:[http:// | + | Crystallization and preliminary crystallographic studies of Saccharomyces cerevisiae alcohol dehydrogenase I., Ramaswamy S, Kratzer DA, Hershey AD, Rogers PH, Arnone A, Eklund H, Plapp BV, J Mol Biol. 1994 Jan 14;235(2):777-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8289298 8289298] |
[[Category: Alcohol dehydrogenase]] | [[Category: Alcohol dehydrogenase]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: ETF]] | [[Category: ETF]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
| - | [[Category: intramolecular disulfide | + | [[Category: intramolecular disulfide bond]] |
[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | [[Category: tetramer of asymmetric | + | [[Category: tetramer of asymmetric dimer]] |
[[Category: zinc coordination]] | [[Category: zinc coordination]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:15:28 2008'' |
Revision as of 15:15, 20 March 2008
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| , resolution 2.44Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | ADH1, ADC1 (Saccharomyces cerevisiae) | ||||||
| Activity: | Alcohol dehydrogenase, with EC number 1.1.1.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Yeast Alcohol Dehydrogenase I, Saccharomyces cerevisiae fermentative enzyme
Overview
The cytoplasmic yeast alcohol dehydrogenase I crystallized at 5 degrees C as hexagonal plates or short columns in the presence of NAD+ and 2,2,2-trifluoroethanol, in sodium N-tris(hydroxymethyl)methyl-3-aminopropanesulfonate buffer at pH 8.2 to 8.6, using polyethylene glycol 4000 as precipitant. X-ray diffraction data to 3.2 A resolution show that the crystals are hexagonal in space group P622 with unit cell dimensions a = b = 147.9 A, c = 69.1 A. There is one subunit of the tetrameric enzyme per asymmetric unit, giving a packing density of 2.9 A3/Da.
About this Structure
2HCY is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary crystallographic studies of Saccharomyces cerevisiae alcohol dehydrogenase I., Ramaswamy S, Kratzer DA, Hershey AD, Rogers PH, Arnone A, Eklund H, Plapp BV, J Mol Biol. 1994 Jan 14;235(2):777-9. PMID:8289298
Page seeded by OCA on Thu Mar 20 17:15:28 2008
