2het
From Proteopedia
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| - | [[Image:2het.gif|left|200px]] | + | [[Image:2het.gif|left|200px]] |
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| - | '''Non-myristoylated bovine recoverin (truncated at C-terminus) with calcium bound to EF-hand 3''' | + | {{Structure |
| + | |PDB= 2het |SIZE=350|CAPTION= <scene name='initialview01'>2het</scene>, resolution 3.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= RCV1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus]) | ||
| + | }} | ||
| + | |||
| + | '''Non-myristoylated bovine recoverin (truncated at C-terminus) with calcium bound to EF-hand 3''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HET is a [ | + | 2HET is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HET OCA]. |
==Reference== | ==Reference== | ||
| - | Tuning of a neuronal calcium sensor., Weiergraber OH, Senin II, Zernii EY, Churumova VA, Kovaleva NA, Nazipova AA, Permyakov SE, Permyakov EA, Philippov PP, Granzin J, Koch KW, J Biol Chem. 2006 Dec 8;281(49):37594-602. Epub 2006 Oct 2. PMID:[http:// | + | Tuning of a neuronal calcium sensor., Weiergraber OH, Senin II, Zernii EY, Churumova VA, Kovaleva NA, Nazipova AA, Permyakov SE, Permyakov EA, Philippov PP, Granzin J, Koch KW, J Biol Chem. 2006 Dec 8;281(49):37594-602. Epub 2006 Oct 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17015448 17015448] |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: recoverin]] | [[Category: recoverin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:16:07 2008'' |
Revision as of 15:16, 20 March 2008
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| , resolution 3.0Å | |||||||
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| Ligands: | |||||||
| Gene: | RCV1 (Bos taurus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Non-myristoylated bovine recoverin (truncated at C-terminus) with calcium bound to EF-hand 3
Overview
Recoverin is a Ca(2+)-regulated signal transduction modulator expressed in the vertebrate retina that has been implicated in visual adaptation. An intriguing feature of recoverin is a cluster of charged residues at its C terminus, the functional significance of which is largely unclear. To elucidate the impact of this segment on recoverin structure and function, we have investigated a mutant lacking the C-terminal 12 amino acids. Whereas in myristoylated recoverin the truncation causes an overall decrease in Ca(2+) sensitivity, results for the non-myristoylated mutant indicate that the truncation primarily affects the high affinity EF-hand 3. The three-dimensional structure of the mutant has been determined by x-ray crystallography. In addition to significant changes in average coordinates compared with wild-type recoverin, the structure provides strong indication of increased conformational flexibility, particularly in the C-terminal domain. Based on these observations, we propose a novel role of the C-terminal segment of recoverin as an internal modulator of Ca(2+) sensitivity.
About this Structure
2HET is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Tuning of a neuronal calcium sensor., Weiergraber OH, Senin II, Zernii EY, Churumova VA, Kovaleva NA, Nazipova AA, Permyakov SE, Permyakov EA, Philippov PP, Granzin J, Koch KW, J Biol Chem. 2006 Dec 8;281(49):37594-602. Epub 2006 Oct 2. PMID:17015448
Page seeded by OCA on Thu Mar 20 17:16:07 2008
