2hhk
From Proteopedia
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| - | [[Image:2hhk.gif|left|200px]] | + | [[Image:2hhk.gif|left|200px]] |
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| - | '''Reaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with dibrominated phosphatidylglycerol''' | + | {{Structure |
| + | |PDB= 2hhk |SIZE=350|CAPTION= <scene name='initialview01'>2hhk</scene>, resolution 2.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=PGT:(1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL+STEARATE'>PGT</scene>, <scene name='pdbligand=PGK:(1R)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL+(9S,10S)-9,10-DIBROMOOCTADECANOATE'>PGK</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Reaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with dibrominated phosphatidylglycerol''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HHK is a [ | + | 2HHK is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HHK OCA]. |
==Reference== | ==Reference== | ||
| - | Brominated lipids identify lipid binding sites on the surface of the reaction center from Rhodobacter sphaeroides., Roszak AW, Gardiner AT, Isaacs NW, Cogdell RJ, Biochemistry. 2007 Mar 20;46(11):2909-16. Epub 2007 Feb 23. PMID:[http:// | + | Brominated lipids identify lipid binding sites on the surface of the reaction center from Rhodobacter sphaeroides., Roszak AW, Gardiner AT, Isaacs NW, Cogdell RJ, Biochemistry. 2007 Mar 20;46(11):2909-16. Epub 2007 Feb 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17315985 17315985] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Rhodobacter sphaeroides]] | [[Category: Rhodobacter sphaeroides]] | ||
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[[Category: U10]] | [[Category: U10]] | ||
[[Category: brominated lipid]] | [[Category: brominated lipid]] | ||
| - | [[Category: lipid binding | + | [[Category: lipid binding site]] |
[[Category: membrane protein]] | [[Category: membrane protein]] | ||
[[Category: photosynthesis]] | [[Category: photosynthesis]] | ||
[[Category: photosynthetic reaction center]] | [[Category: photosynthetic reaction center]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:17:14 2008'' |
Revision as of 15:17, 20 March 2008
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| , resolution 2.50Å | |||||||
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| Ligands: | , , , , , , , , , , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Reaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with dibrominated phosphatidylglycerol
Overview
This study describes the use of brominated phospholipids to distinguish between lipid and detergent binding sites on the surface of a typical alpha-helical membrane protein. Reaction centers isolated from Rhodobacter sphaeroides were cocrystallized with added brominated phospholipids. X-ray structural analysis of these crystals has revealed the presence of two lipid binding sites from the characteristic strong X-ray scattering from the bromine atoms. These results demonstrate the usefulness of this approach to mapping lipid binding sites at the surface of membrane proteins.
About this Structure
2HHK is a Protein complex structure of sequences from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.
Reference
Brominated lipids identify lipid binding sites on the surface of the reaction center from Rhodobacter sphaeroides., Roszak AW, Gardiner AT, Isaacs NW, Cogdell RJ, Biochemistry. 2007 Mar 20;46(11):2909-16. Epub 2007 Feb 23. PMID:17315985
Page seeded by OCA on Thu Mar 20 17:17:14 2008
Categories: Protein complex | Rhodobacter sphaeroides | Cogdell, R J. | Gardiner, A T. | Isaacs, N W. | Roszak, A W. | BCL | BPH | CDL | CL | FE | GOL | K | LDA | PGK | PGT | PO4 | U10 | Brominated lipid | Lipid binding site | Membrane protein | Photosynthesis | Photosynthetic reaction center
