2hi5
From Proteopedia
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| - | [[Image:2hi5.jpg|left|200px]] | + | [[Image:2hi5.jpg|left|200px]] |
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| - | '''Model for bacteriophage fd from cryo-EM''' | + | {{Structure |
| + | |PDB= 2hi5 |SIZE=350|CAPTION= <scene name='initialview01'>2hi5</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Model for bacteriophage fd from cryo-EM''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HI5 is a [ | + | 2HI5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phage_phi3t Phage phi3t]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HI5 OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of a filamentous bacteriophage., Wang YA, Yu X, Overman S, Tsuboi M, Thomas GJ Jr, Egelman EH, J Mol Biol. 2006 Aug 11;361(2):209-15. Epub 2006 Jun 30. PMID:[http:// | + | The structure of a filamentous bacteriophage., Wang YA, Yu X, Overman S, Tsuboi M, Thomas GJ Jr, Egelman EH, J Mol Biol. 2006 Aug 11;361(2):209-15. Epub 2006 Jun 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16843489 16843489] |
[[Category: Phage phi3t]] | [[Category: Phage phi3t]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: structural protein]] | [[Category: structural protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:17:29 2008'' |
Revision as of 15:17, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Model for bacteriophage fd from cryo-EM
Overview
Many thin helical polymers, including bacterial pili and filamentous bacteriophage, have been seen as refractory to high-resolution studies by electron microscopy. Studies of the quaternary structure of such filaments have depended upon techniques such as modeling or X-ray fiber diffraction, given that direct visualization of the subunit organization has not been possible. We report the first image reconstruction of a filamentous virus, bacteriophage fd, by cryoelectron microscopy. Although these thin ( approximately 70 A in diameter) rather featureless filaments scatter weakly, we have been able to achieve a nominal resolution of approximately 8 A using an iterative helical reconstruction procedure. We show that two different conformations of the virus exist, and that in both states the subunits are packed differently than in conflicting models previously proposed on the basis of X-ray fiber diffraction or solid-state NMR studies. A significant fraction of the population of wild-type fd is either disordered or in multiple conformational states, while in the presence of the Y21M mutation, this heterogeneity is greatly reduced, consistent with previous observations. These results show that new computational approaches to helical reconstruction can greatly extend the ability to visualize heterogeneous protein polymers at a reasonably high resolution.
About this Structure
2HI5 is a Single protein structure of sequence from Phage phi3t. Full crystallographic information is available from OCA.
Reference
The structure of a filamentous bacteriophage., Wang YA, Yu X, Overman S, Tsuboi M, Thomas GJ Jr, Egelman EH, J Mol Biol. 2006 Aug 11;361(2):209-15. Epub 2006 Jun 30. PMID:16843489
Page seeded by OCA on Thu Mar 20 17:17:29 2008
