This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3uno

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{STRUCTURE_3uno| PDB=3uno | SCENE= }}
+
==Mycobacterium tuberculosis ferritin homolog, BfrB==
-
===Mycobacterium tuberculosis ferritin homolog, BfrB===
+
<StructureSection load='3uno' size='340' side='right' caption='[[3uno]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-
{{ABSTRACT_PUBMED_21139218}}
+
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3uno]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UNO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UNO FirstGlance]. <br>
 +
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3oj5|3oj5]]</td></tr>
 +
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bfrB, ftn, MT3949 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uno OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uno RCSB], [http://www.ebi.ac.uk/pdbsum/3uno PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Mycobacterium tuberculosis (Mtb) is the causative agent of the deadly disease tuberculosis. Iron acquisition, regulation and storage are critical for the survival of this pathogen within a host. Thus, understanding the mechanisms of iron metabolism in Mtb will shed light on its pathogenic nature, as iron is important for infection. Ferritins are a superfamily of protein nanocages that function in both iron detoxification and storage, and Mtb contains both a predicted ferritin and a bacterioferritin. Here, the cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the ferritin homolog (Mtb BfrB, Rv3841) is reported. An Mtb BfrB crystal grown at pH 6.5 using the hanging-drop vapor-diffusion technique diffracted to 2.50 A resolution and belonged to space group C2, with unit-cell parameters a=226.2, b=226.8, c=113.7 A, beta=94.7 degrees and with 24 subunits per asymmetric unit. Furthermore, modeling the crystal structure of a homologous ferritin into a low-resolution small-angle X-ray scattering (SAXS) electron-density envelope is consistent with the presence of 24 subunits in the BfrB protein cage quaternary structure.
-
==Function==
+
Crystallization and preliminary X-ray crystallographic analysis of a Mycobacterium tuberculosis ferritin homolog, BfrB.,McMath LM, Habel JE, Sankaran B, Yu M, Hung LW, Goulding CW Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 1;66(Pt 12):1657-61., doi: 10.1107/S1744309110042958. Epub 2010 Nov 26. PMID:21139218<ref>PMID:21139218</ref>
-
[[http://www.uniprot.org/uniprot/BFRB_MYCTU BFRB_MYCTU]] Iron-storage protein that displays ferroxidase activity, catalyzing the oxidation of Fe(2+) ions into Fe(3+) ions, that can then be deposited as a ferric-oxide mineral core within the central cavity of the protein complex.<ref>PMID:21494619</ref>
+
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[3uno]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UNO OCA].
+
</div>
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:021139218</ref><references group="xtra"/><references/>
+
__TOC__
 +
</StructureSection>
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
-
[[Category: Contreras, H.]]
+
[[Category: Contreras, H]]
-
[[Category: Goulding, C W.]]
+
[[Category: Goulding, C W]]
-
[[Category: McMath, L M.]]
+
[[Category: McMath, L M]]
-
[[Category: TBSGC, TB Structural Genomics Consortium.]]
+
[[Category: Structural genomic]]
[[Category: Ferritin]]
[[Category: Ferritin]]
[[Category: Ferroxidase activity]]
[[Category: Ferroxidase activity]]
[[Category: Iron storage]]
[[Category: Iron storage]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]

Revision as of 06:27, 21 December 2014

Mycobacterium tuberculosis ferritin homolog, BfrB

3uno, resolution 2.50Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3uno"
Personal tools