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4jyp

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{{STRUCTURE_4jyp| PDB=4jyp | SCENE= }}
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==crystal Structure of KAI2 Apo form==
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===crystal Structure of KAI2 Apo form===
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<StructureSection load='4jyp' size='340' side='right' caption='[[4jyp]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23613584}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4jyp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JYP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JYP FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4jym|4jym]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AT4g37470, F6G17.120, KAI2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jyp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jyp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jyp RCSB], [http://www.ebi.ac.uk/pdbsum/4jyp PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Genetic studies in Arabidopsis implicate an alpha/beta-hydrolase, KARRIKIN-INSENSITIVE 2 (KAI2) as a receptor for karrikins, germination-promoting butenolide small molecules found in the smoke of burned plants. However, direct biochemical evidence for the interaction between KAI2 and karrikin and for the mechanism of downstream signaling by a KAI2-karrikin complex remain elusive. We report crystallographic analyses and ligand-binding experiments for KAI2 recognition of karrikins. The karrikin-1 (KAR1) ligand sits in the opening to the active site abutting a helical domain insert but distal from the canonical catalytic triad (Ser95-His246-Asp217) of alpha/beta-hydrolases, consistent with the lack of detectable hydrolytic activity by purified KAI2. The closest approach of KAR1 to Ser95-His246-Asp217 is 3.8 A from His246. Six aromatic side chains, including His246, encapsulate KAR1 through geometrically defined aromatic-aromatic interactions. KAR1 binding induces a conformational change in KAI2 at the active site entrance. A crevice of hydrophobic residues linking the polar edge of KAR1 and the helical domain insert suggests that KAI2-KAR1 creates a contiguous interface for binding signaling partners in a ligand-dependent manner.
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==Function==
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Smoke-derived karrikin perception by the alpha/beta-hydrolase KAI2 from Arabidopsis.,Guo Y, Zheng Z, La Clair JJ, Chory J, Noel JP Proc Natl Acad Sci U S A. 2013 Apr 23. PMID:23613584<ref>PMID:23613584</ref>
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[[http://www.uniprot.org/uniprot/KAI2_ARATH KAI2_ARATH]] Involved in seed germination and seedling development. Essential for plant responses to karrikins, a class of butenolide compounds, structurally similar to strigolactones, released from burning vegetation that stimulate seed germination and enhance seedling photomorphogenesis. KAI2 is not required for strigolactone-mediated responses, but MAX2 is necessary for responses to karrikins and strigolactones.<ref>PMID:20864454</ref> <ref>PMID:22357928</ref> <ref>PMID:23142794</ref> <ref>PMID:23301669</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4jyp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JYP OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:023613584</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
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[[Category: Guo, Y.]]
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[[Category: Guo, Y]]
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[[Category: Noel, J P.]]
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[[Category: Noel, J P]]
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[[Category: Zheng, Z.]]
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[[Category: Zheng, Z]]
[[Category: Alpha/beta hydrolase]]
[[Category: Alpha/beta hydrolase]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]

Revision as of 15:17, 21 December 2014

crystal Structure of KAI2 Apo form

4jyp, resolution 1.30Å

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