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2quo

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{{STRUCTURE_2quo| PDB=2quo | SCENE= }}
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==Crystal Structure of C terminal fragment of Clostridium perfringens enterotoxin==
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===Crystal Structure of C terminal fragment of Clostridium perfringens enterotoxin===
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<StructureSection load='2quo' size='340' side='right' caption='[[2quo]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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{{ABSTRACT_PUBMED_17977833}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2quo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QUO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QUO FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CPE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1502 Clostridium perfringens])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2quo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2quo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2quo RCSB], [http://www.ebi.ac.uk/pdbsum/2quo PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Clostridium perfringens enterotoxin is a common cause of food-borne and antibiotic-associated diarrhea. The toxin's receptors on intestinal epithelial cells include claudin-3 and -4, members of a large family of tight junction proteins. Toxin-induced cytolytic pore formation requires residues in the NH(2)-terminal half, whereas residues near the COOH terminus are required for binding to claudins. The claudin-binding COOH-terminal domain is not toxic and is currently under investigation as a potential drug absorption enhancer. Because claudin-4 is overexpressed on some human cancers, the toxin is also being investigated for targeting chemotherapy. Our aim was to solve the structure of the claudin-binding domain to advance its therapeutic applications. The structure of a 14-kDa fragment containing residues 194 to the native COOH terminus at position 319 was solved by x-ray diffraction to a resolution of 1.75A. The structure is a nine-strand beta sandwich with previously unappreciated similarity to the receptor-binding domains of several other toxins of spore-forming bacteria, including the collagen-binding domain of ColG from Clostridium histolyticum and the large Cry family of toxins (including Cry4Ba) of Bacillus thuringiensis. Correlations with previous studies suggest that the claudin-4 binding site is on a large surface loop between strands beta8 and beta9 or includes these strands. The sequence that was crystallized (residues 194-319) binds to purified human claudin-4 with a 1:1 stoichiometry and affinity in the submicromolar range similar to that observed for binding of native toxin to cells. Our results provide a structural framework to advance therapeutic applications of the toxin and suggest a common ancestor for several receptor-binding domains of bacterial toxins.
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==Function==
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Structure of the claudin-binding domain of Clostridium perfringens enterotoxin.,Van Itallie CM, Betts L, Smedley JG 3rd, McClane BA, Anderson JM J Biol Chem. 2008 Jan 4;283(1):268-74. Epub 2007 Oct 31. PMID:17977833<ref>PMID:17977833</ref>
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[[http://www.uniprot.org/uniprot/ELTB_CLOPF ELTB_CLOPF]] This enterotoxin is responsible for many cases of a mild type of food poisoning.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[2quo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QUO OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:017977833</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Clostridium perfringens]]
[[Category: Clostridium perfringens]]
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[[Category: Betts, L.]]
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[[Category: Betts, L]]
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[[Category: Itallie, C M.Van.]]
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[[Category: Itallie, C M.Van]]
[[Category: Beta sandwich]]
[[Category: Beta sandwich]]
[[Category: Claudin-4 binding domain]]
[[Category: Claudin-4 binding domain]]

Revision as of 12:30, 18 December 2014

Crystal Structure of C terminal fragment of Clostridium perfringens enterotoxin

2quo, resolution 1.75Å

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