We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

4dcn

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{STRUCTURE_4dcn| PDB=4dcn | SCENE= }}
+
==Crystal Structure Analysis of the Arfaptin2 BAR domain in Complex with ARL1==
-
===Crystal Structure Analysis of the Arfaptin2 BAR domain in Complex with ARL1===
+
<StructureSection load='4dcn' size='340' side='right' caption='[[4dcn]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
-
{{ABSTRACT_PUBMED_22679020}}
+
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[4dcn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DCN FirstGlance]. <br>
 +
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 +
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1moz|1moz]], [[1upt|1upt]], [[1i49|1i49]], [[1i4l|1i4l]], [[3dyt|3dyt]]</td></tr>
 +
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ARL1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), ARFIP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dcn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dcn RCSB], [http://www.ebi.ac.uk/pdbsum/4dcn PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Membrane-sculpting BAR (Bin/Amphiphysin/Rvs) domains form a crescent-shaped homodimer that can sense and induce membrane curvature through its positively charged concave face. We have recently shown that Arfaptin-2, which was originally identified as a binding partner for the Arf and Rac1 GTPases, binds to Arl1 through its BAR domain and is recruited onto Golgi membranes. There, Arfaptin-2 induces membrane tubules. Here, we report the crystal structure of the Arfaptin-2 BAR homodimer in complex with two Arl1 molecules bound symmetrically to each side, leaving the concave face open for membrane association. The overall structure of the Arl1.Arfaptin-2 BAR complex closely resembles that of the PX-BAR domain of sorting nexin 9, suggesting similar mechanisms underlying BAR domain targeting to specific organellar membranes. The Arl1.Arfaptin-2 BAR structure suggests that one of the two Arl1 molecules competes with Rac1, which binds to the concave face of the Arfaptin-2 BAR homodimer and may hinder its membrane association.
-
==Function==
+
Structural basis for membrane binding specificity of the Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin-2 determined by Arl1 GTPase.,Nakamura K, Man Z, Xie Y, Hanai A, Makyio H, Kawasaki M, Kato R, Shin HW, Nakayama K, Wakatsuki S J Biol Chem. 2012 Jul 20;287(30):25478-89. doi: 10.1074/jbc.M112.365783. Epub, 2012 Jun 7. PMID:22679020<ref>PMID:22679020</ref>
-
[[http://www.uniprot.org/uniprot/ARL1_HUMAN ARL1_HUMAN]] GTP-binding protein that has very low efficiency as allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Can activate phospholipase D with very low efficiency. Important for normal function of the Golgi apparatus.<ref>PMID:9624189</ref> [[http://www.uniprot.org/uniprot/ARFP2_HUMAN ARFP2_HUMAN]] Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling.
+
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[4dcn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DCN OCA].
+
</div>
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:022679020</ref><references group="xtra"/><references/>
+
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Kato, R.]]
+
[[Category: Kato, R]]
-
[[Category: Kawasaki, M.]]
+
[[Category: Kawasaki, M]]
-
[[Category: Nakamura, K.]]
+
[[Category: Nakamura, K]]
-
[[Category: Wakatsuki, S.]]
+
[[Category: Wakatsuki, S]]
-
[[Category: Xie, Y.]]
+
[[Category: Xie, Y]]
[[Category: Bar domain]]
[[Category: Bar domain]]
[[Category: Membrane deformation]]
[[Category: Membrane deformation]]
[[Category: Protein binding-signaling protein complex]]
[[Category: Protein binding-signaling protein complex]]
[[Category: Small gtpase effector complex]]
[[Category: Small gtpase effector complex]]

Revision as of 09:21, 21 December 2014

Crystal Structure Analysis of the Arfaptin2 BAR domain in Complex with ARL1

4dcn, resolution 3.01Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4dcn"
Personal tools