2i21
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2i21.gif|left|200px]] | + | [[Image:2i21.gif|left|200px]] |
| - | + | ||
| - | '''Bacteriorhodopsin/lipid complex, T46V mutant''' | + | {{Structure |
| + | |PDB= 2i21 |SIZE=350|CAPTION= <scene name='initialview01'>2i21</scene>, resolution 1.84Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=RET:RETINAL'>RET</scene>, <scene name='pdbligand=LI1:1-[2,6,10.14-TETRAMETHYL-HEXADECAN-16-YL]-2-[2,10,14-TRIMETHYLHEXADECAN-16-YL]GLYCEROL'>LI1</scene> and <scene name='pdbligand=SQU:2,10,23-TRIMETHYL-TETRACOSANE'>SQU</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Bacteriorhodopsin/lipid complex, T46V mutant''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2I21 is a [ | + | 2I21 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I21 OCA]. |
==Reference== | ==Reference== | ||
| - | Propagating structural perturbation inside bacteriorhodopsin: crystal structures of the M state and the D96A and T46V mutants., Lanyi JK, Schobert B, Biochemistry. 2006 Oct 3;45(39):12003-10. PMID:[http:// | + | Propagating structural perturbation inside bacteriorhodopsin: crystal structures of the M state and the D96A and T46V mutants., Lanyi JK, Schobert B, Biochemistry. 2006 Oct 3;45(39):12003-10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17002299 17002299] |
[[Category: Halobacterium salinarum]] | [[Category: Halobacterium salinarum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 22: | Line 31: | ||
[[Category: ion pump]] | [[Category: ion pump]] | ||
[[Category: ion transport]] | [[Category: ion transport]] | ||
| - | [[Category: | + | [[Category: lipid]] |
[[Category: membrane protein]] | [[Category: membrane protein]] | ||
[[Category: photoreceptor]] | [[Category: photoreceptor]] | ||
| Line 28: | Line 37: | ||
[[Category: serpentine]] | [[Category: serpentine]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:24:30 2008'' |
Revision as of 15:24, 20 March 2008
| |||||||
| , resolution 1.84Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Bacteriorhodopsin/lipid complex, T46V mutant
Overview
The X-ray diffraction structure of the non-illuminated D96A bacteriorhodopsin mutant reveals structural changes as far away as 15 A from residue 96, at the retinal, Trp-182, Ala-215, and waters 501, 402, and 401. The Asp-to-Ala side-chain replacement breaks its hydrogen bond with Thr-46, and the resulting separation of the cytoplasmic ends of helices B and C is communicated to the retinal region through a chain of covalent and hydrogen bonds. The unexpected long-range consequences of the D96A mutation include breaking the hydrogen bond between O of Ala-215 and water 501 and the formation of a new hydrogen bond between water molecules 401 and 402 in the extracellular region. Because in the T46V mutant a new water molecule appears at Asp-96 and its hydrogen-bond to Ile-45 replaces Thr-46 as its link to helix B, the separation of helices B and C is smaller than that in D96A, and there are no atomic displacements elsewhere in the protein. Propagation of conformational changes along the chain between the retinal and Thr-46 had been observed earlier in the crystal structures of the D96N and E204Q mutants but in the trapped M state. Consistent with the perturbation of the retinal region in D96A, little change of the Thr-46 region occurs between the non-illuminated and M states of this mutant. It appears that a local perturbation can propagate along a track in both directions between the retinal and the Asp-96/Thr-46 pair, either from photoisomerization of the retinal in the wild-type protein in one case or from the D96A mutation in the other.
About this Structure
2I21 is a Single protein structure of sequence from Halobacterium salinarum. Full crystallographic information is available from OCA.
Reference
Propagating structural perturbation inside bacteriorhodopsin: crystal structures of the M state and the D96A and T46V mutants., Lanyi JK, Schobert B, Biochemistry. 2006 Oct 3;45(39):12003-10. PMID:17002299
Page seeded by OCA on Thu Mar 20 17:24:30 2008
Categories: Halobacterium salinarum | Single protein | Lanyi, J K. | Schobert, B. | LI1 | RET | SQU | 7-transmembrane | Haloarchaea | Ion pump | Ion transport | Lipid | Membrane protein | Photoreceptor | Retinal protein | Serpentine
