2i5o
From Proteopedia
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| - | [[Image:2i5o.jpg|left|200px]]< | + | [[Image:2i5o.jpg|left|200px]] |
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| - | '''Solution Structure of the Ubiquitin-Binding Zinc Finger (UBZ) Domain of the Human DNA Y-Polymerase Eta''' | + | {{Structure |
| + | |PDB= 2i5o |SIZE=350|CAPTION= <scene name='initialview01'>2i5o</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] | ||
| + | |GENE= POLH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Solution Structure of the Ubiquitin-Binding Zinc Finger (UBZ) Domain of the Human DNA Y-Polymerase Eta''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2I5O is a [ | + | 2I5O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I5O OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the ubiquitin-binding zinc finger domain of human DNA Y-polymerase eta., Bomar MG, Pai MT, Tzeng SR, Li SS, Zhou P, EMBO Rep. 2007 Mar;8(3):247-51. Epub 2007 Feb 16. PMID:[http:// | + | Structure of the ubiquitin-binding zinc finger domain of human DNA Y-polymerase eta., Bomar MG, Pai MT, Tzeng SR, Li SS, Zhou P, EMBO Rep. 2007 Mar;8(3):247-51. Epub 2007 Feb 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17304240 17304240] |
[[Category: DNA-directed DNA polymerase]] | [[Category: DNA-directed DNA polymerase]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: zinc finger]] | [[Category: zinc finger]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:25:48 2008'' |
Revision as of 15:25, 20 March 2008
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| Ligands: | |||||||
| Gene: | POLH (Homo sapiens) | ||||||
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of the Ubiquitin-Binding Zinc Finger (UBZ) Domain of the Human DNA Y-Polymerase Eta
Contents |
Overview
The ubiquitin-binding zinc finger (UBZ) domain of human DNA Y-family polymerase (pol) eta is important in the recruitment of the polymerase to the stalled replication machinery in translesion synthesis. Here, we report the solution structure of the pol eta UBZ domain and its interaction with ubiquitin. We show that the UBZ domain adopts a classical C(2)H(2) zinc-finger structure characterized by a betabetaalpha fold. Nuclear magnetic resonance titration maps the binding interfaces between UBZ and ubiquitin to the alpha-helix of the UBZ domain and the canonical hydrophobic surface of ubiquitin defined by residues L8, I44 and V70. Although the UBZ domain binds ubiquitin through a single alpha-helix, in a manner similar to the inverted ubiquitin-interacting motif, its structure is distinct from previously characterized ubiquitin-binding domains. The pol eta UBZ domain represents a novel member of the C(2)H(2) zinc finger family that interacts with ubiquitin to regulate translesion synthesis.
Disease
Known diseases associated with this structure: Xeroderma pigmentosum, variant type OMIM:[603968]
About this Structure
2I5O is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the ubiquitin-binding zinc finger domain of human DNA Y-polymerase eta., Bomar MG, Pai MT, Tzeng SR, Li SS, Zhou P, EMBO Rep. 2007 Mar;8(3):247-51. Epub 2007 Feb 16. PMID:17304240
Page seeded by OCA on Thu Mar 20 17:25:48 2008
