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2i7x

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[[Image:2i7x.jpg|left|200px]]<br /><applet load="2i7x" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2i7x.jpg|left|200px]]
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caption="2i7x, resolution 2.50&Aring;" />
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'''Structure of Yeast CPSF-100 (Ydh1p)'''<br />
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{{Structure
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|PDB= 2i7x |SIZE=350|CAPTION= <scene name='initialview01'>2i7x</scene>, resolution 2.50&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE= CFT2, YDH1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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}}
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'''Structure of Yeast CPSF-100 (Ydh1p)'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2I7X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I7X OCA].
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2I7X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I7X OCA].
==Reference==
==Reference==
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Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease., Mandel CR, Kaneko S, Zhang H, Gebauer D, Vethantham V, Manley JL, Tong L, Nature. 2006 Dec 14;444(7121):953-6. Epub 2006 Nov 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17128255 17128255]
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Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease., Mandel CR, Kaneko S, Zhang H, Gebauer D, Vethantham V, Manley JL, Tong L, Nature. 2006 Dec 14;444(7121):953-6. Epub 2006 Nov 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17128255 17128255]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Tong, L.]]
[[Category: Tong, L.]]
[[Category: Zhang, H.]]
[[Category: Zhang, H.]]
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[[Category: artemis]]
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[[Category: artemi]]
[[Category: double-strand break repair]]
[[Category: double-strand break repair]]
[[Category: metallo-b-lactamase]]
[[Category: metallo-b-lactamase]]
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[[Category: v(d)j recombination]]
[[Category: v(d)j recombination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:50:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:26:30 2008''

Revision as of 15:26, 20 March 2008

Image:2i7x.jpg


PDB ID 2i7x

Drag the structure with the mouse to rotate
, resolution 2.50Å
Gene: CFT2, YDH1 (Saccharomyces cerevisiae)
Coordinates: save as pdb, mmCIF, xml



Structure of Yeast CPSF-100 (Ydh1p)


Overview

Most eukaryotic messenger RNA precursors (pre-mRNAs) undergo extensive maturational processing, including cleavage and polyadenylation at the 3'-end. Despite the characterization of many proteins that are required for the cleavage reaction, the identity of the endonuclease is not known. Recent analyses indicated that the 73-kDa subunit of cleavage and polyadenylation specificity factor (CPSF-73) might be the endonuclease for this and related reactions, although no direct data confirmed this. Here we report the crystal structures of human CPSF-73 at 2.1 A resolution, complexed with zinc ions and a sulphate that might mimic the phosphate group of the substrate, and the related yeast protein CPSF-100 (Ydh1) at 2.5 A resolution. Both CPSF-73 and CPSF-100 contain two domains, a metallo-beta-lactamase domain and a novel beta-CASP (named for metallo-beta-lactamase, CPSF, Artemis, Snm1, Pso2) domain. The active site of CPSF-73, with two zinc ions, is located at the interface of the two domains. Purified recombinant CPSF-73 possesses RNA endonuclease activity, and mutations that disrupt zinc binding in the active site abolish this activity. Our studies provide the first direct experimental evidence that CPSF-73 is the pre-mRNA 3'-end-processing endonuclease.

About this Structure

2I7X is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease., Mandel CR, Kaneko S, Zhang H, Gebauer D, Vethantham V, Manley JL, Tong L, Nature. 2006 Dec 14;444(7121):953-6. Epub 2006 Nov 26. PMID:17128255

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