2ibz
From Proteopedia
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| - | [[Image:2ibz.jpg|left|200px]] | + | [[Image:2ibz.jpg|left|200px]] |
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| - | '''Yeast Cytochrome BC1 Complex with Stigmatellin''' | + | {{Structure |
| + | |PDB= 2ibz |SIZE=350|CAPTION= <scene name='initialview01'>2ibz</scene>, resolution 2.3Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=UQ6:5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL'>UQ6</scene> and <scene name='pdbligand=SMA:STIGMATELLIN A'>SMA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Ubiquinol--cytochrome-c_reductase Ubiquinol--cytochrome-c reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.2.2 1.10.2.2] | ||
| + | |GENE= variable domain antibody heavy chain ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), variable domain antibody light chain ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | ||
| + | }} | ||
| + | |||
| + | '''Yeast Cytochrome BC1 Complex with Stigmatellin''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2IBZ is a [ | + | 2IBZ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IBZ OCA]. |
==Reference== | ==Reference== | ||
| - | A comparison of stigmatellin conformations, free and bound to the photosynthetic reaction center and the cytochrome bc1 complex., Lancaster CR, Hunte C, Kelley J 3rd, Trumpower BL, Ditchfield R, J Mol Biol. 2007 Apr 20;368(1):197-208. Epub 2007 Feb 11. PMID:[http:// | + | A comparison of stigmatellin conformations, free and bound to the photosynthetic reaction center and the cytochrome bc1 complex., Lancaster CR, Hunte C, Kelley J 3rd, Trumpower BL, Ditchfield R, J Mol Biol. 2007 Apr 20;368(1):197-208. Epub 2007 Feb 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17337272 17337272] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: multisubunit membrane protein complex]] | [[Category: multisubunit membrane protein complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:27:40 2008'' |
Revision as of 15:27, 20 March 2008
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| , resolution 2.3Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Gene: | variable domain antibody heavy chain (Mus musculus), variable domain antibody light chain (Mus musculus) | ||||||
| Activity: | Ubiquinol--cytochrome-c reductase, with EC number 1.10.2.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Yeast Cytochrome BC1 Complex with Stigmatellin
Overview
We describe in detail the conformations of the inhibitor stigmatellin in its free form and bound to the ubiquinone-reducing (Q(B)) site of the reaction center and to the ubiquinol-oxidizing (Q(o)) site of the cytochrome bc(1) complex. We present here the first structures of a stereochemically correct stigmatellin in complexes with a bacterial reaction center and the yeast cytochrome bc1 complex. The conformations of the inhibitor bound to the two enzymes are not the same. We focus on the orientations of the stigmatellin side-chain relative to the chromone head group, and on the interaction of the stigmatellin side-chain with these membrane protein complexes. The different conformations of stigmatellin found illustrate the structural variability of the Q sites, which are affected by the same inhibitor. The free rotation about the chi1 dihedral angle is an essential factor for allowing stigmatellin to bind in both the reaction center and the cytochrome bc1 pocket.
About this Structure
2IBZ is a Protein complex structure of sequences from Mus musculus and Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
A comparison of stigmatellin conformations, free and bound to the photosynthetic reaction center and the cytochrome bc1 complex., Lancaster CR, Hunte C, Kelley J 3rd, Trumpower BL, Ditchfield R, J Mol Biol. 2007 Apr 20;368(1):197-208. Epub 2007 Feb 11. PMID:17337272
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