4gy4
From Proteopedia
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| - | + | ==Role of the biradical intermediate observed during the turnover of SLAC: A two-domain laccase from Streptomyces coelicolor== | |
| - | + | <StructureSection load='4gy4' size='340' side='right' caption='[[4gy4]], [[Resolution|resolution]] 2.67Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4gy4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor_a3(2) Streptomyces coelicolor a3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GY4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GY4 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4gxf|4gxf]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SCO6712 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 Streptomyces coelicolor A3(2)])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gy4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gy4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gy4 RCSB], [http://www.ebi.ac.uk/pdbsum/4gy4 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The enzyme mechanism of the multicopper oxidase (MCO) SLAC from Streptomyces coelicolor was investigated by structural (XRD), spectroscopic (optical, EPR), and kinetics (stopped-flow) experiments on variants in which residue Tyr108 had been replaced by Phe or Ala through site-directed mutagenesis. Contrary to the more common three-domain MCOs, a tyrosine in the two-domain SLAC is found to participate in the enzyme mechanism by providing an electron during oxygen reduction, giving rise to the temporary appearance of a tyrosyl radical. The relatively low k(cat)/K(M) of SLAC and the involvement of Y108 in the enzyme mechanism may reflect an adaptation to a milieu in which there is an imbalance between the available reducing and oxidizing co-substrates. The purported evolutionary relationship between the two-domain MCOs and human ceruloplasmin appears to extend not only to the 3D structure and the mode of binding of the Cu's in the trinuclear center, as noted before, but also to the enzyme mechanism. | ||
| - | + | Involvement of Tyr108 in the Enzyme Mechanism of the Small Laccase from Streptomyces coelicolor.,Gupta A, Nederlof I, Sottini S, Tepper AW, Groenen EJ, Thomassen EA, Canters GW J Am Chem Soc. 2012 Nov 7;134(44):18213-6. doi: 10.1021/ja3088604. Epub 2012 Oct , 29. PMID:23094962<ref>PMID:23094962</ref> | |
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| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| - | [[Category: Canters, G W | + | == References == |
| - | [[Category: Gupta, A | + | <references/> |
| - | [[Category: Nederlof, I | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Canters, G W]] | ||
| + | [[Category: Gupta, A]] | ||
| + | [[Category: Nederlof, I]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
[[Category: Two-domain lacasse]] | [[Category: Two-domain lacasse]] | ||
Revision as of 11:09, 21 December 2014
Role of the biradical intermediate observed during the turnover of SLAC: A two-domain laccase from Streptomyces coelicolor
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