This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
4huz
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==2,6-Dichloro-p-hydroquinone 1,2-Dioxygenase== | |
| - | ===2,6- | + | <StructureSection load='4huz' size='340' side='right' caption='[[4huz]], [[Resolution|resolution]] 2.60Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4huz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingobium_chlorophenolicum Sphingobium chlorophenolicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HUZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pcpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=46429 Sphingobium chlorophenolicum])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4huz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4huz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4huz RCSB], [http://www.ebi.ac.uk/pdbsum/4huz PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | PcpA (2,6-dichloro-p-hydroquinone 1,2-dioxygenase) from Sphingobium chlorophenolicum, a non-haem Fe(II) dioxygenase capable of cleaving the aromatic ring of p-hydroquinone and its substituted variants, is a member of the recently discovered p-hydroquinone 1,2-dioxygenases. Here we report the 2.6 A structure of PcpA, which consists of four betaalphabetabetabeta motifs, a hallmark of the vicinal oxygen chelate superfamily. The secondary co-ordination sphere of the Fe(II) centre forms an extensive hydrogen-bonding network with three solvent exposed residues, linking the catalytic Fe(II) to solvent. A tight hydrophobic pocket provides p-hydroquinones access to the Fe(II) centre. The p-hydroxyl group is essential for the substrate-binding, thus phenols and catechols, lacking a p-hydroxyl group, do not bind to PcpA. Site-directed mutagenesis and kinetic analysis confirm the critical catalytic role played by the highly conserved His10, Thr13, His226 and Arg259. Based on these results, we propose a general reaction mechanism for p-hydroquinone 1,2-dioxygenases. | ||
| - | + | Structural characterization of 2,6-dichloro-p-hydroquinone 1,2-dioxygenase (PcpA) from Sphingobium chlorophenolicum, a new type of aromatic ring-cleavage enzyme.,Hayes RP, Green AR, Nissen MS, Lewis KM, Xun L, Kang C Mol Microbiol. 2013 Mar 14. doi: 10.1111/mmi.12204. PMID:23489289<ref>PMID:23489289</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Sphingobium chlorophenolicum]] | [[Category: Sphingobium chlorophenolicum]] | ||
| - | [[Category: Green, A R | + | [[Category: Green, A R]] |
| - | [[Category: Hayes, R P | + | [[Category: Hayes, R P]] |
| - | [[Category: Kang, C | + | [[Category: Kang, C]] |
| - | [[Category: Lewis, K M | + | [[Category: Lewis, K M]] |
| - | [[Category: Nissen, M S | + | [[Category: Nissen, M S]] |
| - | [[Category: Xun, L | + | [[Category: Xun, L]] |
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
Revision as of 12:45, 4 January 2015
2,6-Dichloro-p-hydroquinone 1,2-Dioxygenase
| |||||||||||
