4kp3
From Proteopedia
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| - | + | ==Crystal Structure of MyoVa-GTD in Complex with Two Cargos== | |
| - | + | <StructureSection load='4kp3' size='340' side='right' caption='[[4kp3]], [[Resolution|resolution]] 2.41Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[4kp3]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KP3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KP3 FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wb8|3wb8]]</td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Myo5a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), Rilpl2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), Mlph ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kp3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kp3 RCSB], [http://www.ebi.ac.uk/pdbsum/4kp3 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/MYO5A_MOUSE MYO5A_MOUSE]] Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.<ref>PMID:22908308</ref> [[http://www.uniprot.org/uniprot/MELPH_MOUSE MELPH_MOUSE]] Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.<ref>PMID:11887186</ref> [[http://www.uniprot.org/uniprot/RIPL2_MOUSE RIPL2_MOUSE]] Involved in cell shape and neuronal morphogenesis, positively regulating the establishment and maintenance of dendritic spines. May activate RAC1 (By similarity). | [[http://www.uniprot.org/uniprot/MYO5A_MOUSE MYO5A_MOUSE]] Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.<ref>PMID:22908308</ref> [[http://www.uniprot.org/uniprot/MELPH_MOUSE MELPH_MOUSE]] Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.<ref>PMID:11887186</ref> [[http://www.uniprot.org/uniprot/RIPL2_MOUSE RIPL2_MOUSE]] Involved in cell shape and neuronal morphogenesis, positively regulating the establishment and maintenance of dendritic spines. May activate RAC1 (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Class V myosins (MyoV), the most studied unconventional myosins, recognize numerous cargos mainly via the motor's globular tail domain (GTD). Little is known regarding how MyoV-GTD recognizes such a diverse array of cargos specifically. Here, we solved the crystal structures of MyoVa-GTD in its apo-form and in complex with two distinct cargos, melanophilin and Rab interacting lysosomal protein-like 2. The apo-MyoVa-GTD structure indicates that most mutations found in patients with Griscelli syndrome, microvillus inclusion disease, or cancers or in "dilute" rodents likely impair the folding of GTD. The MyoVa-GTD/cargo complex structure reveals two distinct cargo-binding surfaces, one primarily via charge-charge interaction and the other mainly via hydrophobic interactions. Structural and biochemical analysis reveal the specific cargo-binding specificities of various isoforms of mammalian MyoV as well as very different cargo recognition mechanisms of MyoV between yeast and higher eukaryotes. The MyoVa-GTD structures resolved here provide a framework for future functional studies of vertebrate class V myosins. | ||
| + | |||
| + | Structural basis of cargo recognitions for class V myosins.,Wei Z, Liu X, Yu C, Zhang M Proc Natl Acad Sci U S A. 2013 Jun 24. PMID:23798443<ref>PMID:23798443</ref> | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | == | + | ==See Also== |
| - | + | *[[Myosin|Myosin]] | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
| - | [[Category: Liu, X | + | [[Category: Liu, X]] |
| - | [[Category: Wei, Z | + | [[Category: Wei, Z]] |
| - | [[Category: Yu, C | + | [[Category: Yu, C]] |
| - | [[Category: Zhang, M | + | [[Category: Zhang, M]] |
[[Category: Helix bundle]] | [[Category: Helix bundle]] | ||
[[Category: Motor protein-protein transport complex]] | [[Category: Motor protein-protein transport complex]] | ||
Revision as of 02:43, 25 December 2014
Crystal Structure of MyoVa-GTD in Complex with Two Cargos
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