TRNA methyltransferase

tRNA methyltransferase (Trm) methylates specific positions on tRNA. tRNA methylation is a post-transcription modification. The methyl donor is S-adenosylmethionine (SAM) and the product is the methylated tRNA and S-adenosylhomocysteine (SAH). S-adenosylornithine or sinefugin (SFG) is an inhibitor of the reaction.

For details on Trm to N2 of guanine 6 see TRNA:m2G6 methyltransferase TrmN/Trm14.

3D structures of tRNA methyltransferase

Updated on 02-January-2014

Trm to N1 of guanine 37

3knu – ApTrm – Anaplasma phagocytophilum

1oy5 – AaTrm – Aquifex aeolicus
3ief – Trm – Bartonella henselae
1uaj – HiTrm – haemophilus influenzae
3ky7 – Trm – Staphylococcus aureus
3quv – Trm – Mycobacterium abscessus

Complexes of Trm to N1 of guanine 37

1uak – HiTrm + SAM

1ual, 1uam – HiTrm + SAH
3axz – HiTrm + adenosine
1p9p – EcTrm + SAH – Escherichia coli
2yx1 – MjTrm + SFG – Methanocalcococcus jannaschii
3ay0 – MjTrm + adenosine
4h3y, 4h3z – Trm + SAH – Burkholderia phymatum
4ig6 – ApTrm + SAH
4gek, 4iwn – EcTrm + SAM derivative

Trm to N7 of guanine 46

1yzh – Trm – Streptococcus pneumoniae

2fca – Trm – Bacillus subtilis
2vdu – Trm – yeast
3dxx – EcTrm

Complexes of Trm to N7 of guanine 46

3ckk – Trm + SAM - human

3dxy – EcTrm + SAM
3dxz – EcTrm + SAH

Trm to O2 of cytidine 34

3e5y – BpTrm – Burkholderia pseudomalllei

Complexes of Trm to O2 of cytidine 34

4kgn – BpTrm + SAH

3n4k, 3n4j – Trm + SAH – Yersinia pestis
1mxi, 1im8 – HiTrm + SAH

Trm to N2 of guanine 6

3tm5 – Trm + SFG – Pyrococcvus furiosus