2ij0

From Proteopedia

Revision as of 15:30, 20 March 2008

Structural basis of T cell specificity and activation by the bacterial superantigen toxic shock syndrome toxin-1

Overview

Superantigens (SAGs) bind simultaneously to major histocompatibility complex (MHC) and T-cell receptor (TCR) molecules, resulting in the massive release of inflammatory cytokines that can lead to toxic shock syndrome (TSS) and death. A major causative agent of TSS is toxic shock syndrome toxin-1 (TSST-1), which is unique relative to other bacterial SAGs owing to its structural divergence and its stringent TCR specificity. Here, we report the crystal structure of TSST-1 in complex with an affinity-matured variant of its wild-type TCR ligand, human T-cell receptor beta chain variable domain 2.1. From this structure and a model of the wild-type complex, we show that TSST-1 engages TCR ligands in a markedly different way than do other SAGs. We provide a structural basis for the high TCR specificity of TSST-1 and present a model of the TSST-1-dependent MHC-SAG-TCR T-cell signaling complex that is structurally and energetically unique relative to those formed by other SAGs. Our data also suggest that protein plasticity plays an exceptionally significant role in this affinity maturation process that results in more than a 3000-fold increase in affinity.

About this Structure

2IJ0 is a Single protein structure of sequence from Homo sapiens and Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

Structural basis of T-cell specificity and activation by the bacterial superantigen TSST-1., Moza B, Varma AK, Buonpane RA, Zhu P, Herfst CA, Nicholson MJ, Wilbuer AK, Seth NP, Wucherpfennig KW, McCormick JK, Kranz DM, Sundberg EJ, EMBO J. 2007 Feb 21;26(4):1187-97. Epub 2007 Feb 1. PMID:17268555

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