4en1

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{{STRUCTURE_4en1| PDB=4en1 | SCENE= }}
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==The 1.62A structure of a FRET-optimized Cerulean Fluorescent Protein==
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===The 1.62A structure of a FRET-optimized Cerulean Fluorescent Protein===
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<StructureSection load='4en1' size='340' side='right' caption='[[4en1]], [[Resolution|resolution]] 1.62&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23633585}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4en1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EN1 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SWG:2-[(4Z)-2-[(1R)-1-AMINO-2-HYDROXY-ETHYL]-4-(1H-INDOL-3-YLMETHYLIDENE)-5-OXO-IMIDAZOL-1-YL]ETHANOIC+ACID'>SWG</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 Aequorea victoria])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4en1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4en1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4en1 RCSB], [http://www.ebi.ac.uk/pdbsum/4en1 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Genetically encoded cyan fluorescent proteins (CFPs) bearing a tryptophan-derived chromophore are commonly used as energy-donor probes in Forster resonance energy transfer (FRET) experiments useful in live cell-imaging applications. In recent years, significant effort has been expended on eliminating the structural and excited-state heterogeneity of these proteins, which has been linked to undesirable photophysical properties. Recently, mCerulean3, a descendant of enhanced CFP, was introduced as an optimized FRET donor protein with a superior quantum yield of 0.87. Here, the 1.6 A resolution X-ray structure of mCerulean3 is reported. The chromophore is shown to adopt a planar trans configuration at low pH values, indicating that the acid-induced isomerization of Cerulean has been eliminated. beta-Strand 7 appears to be well ordered in a single conformation, indicating a loss of conformational heterogeneity in the vicinity of the chromophore. Although the side chains of Ile146 and Leu167 appear to exist in two rotamer states, they are found to be well packed against the indole group of the chromophore. The Ser65 reversion mutation allows improved side-chain packing of Leu220. A structural comparison with mTurquoise2 is presented and additional engineering strategies are discussed.
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==Function==
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The 1.6 A resolution structure of a FRET-optimized Cerulean fluorescent protein.,Watkins JL, Kim H, Markwardt ML, Chen L, Fromme R, Rizzo MA, Wachter RM Acta Crystallogr D Biol Crystallogr. 2013 May;69(Pt 5):767-73. doi:, 10.1107/S0907444913001546. Epub 2013 Apr 11. PMID:23633585<ref>PMID:23633585</ref>
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[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4en1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EN1 OCA].
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</div>
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==Reference==
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==See Also==
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<ref group="xtra">PMID:023633585</ref><references group="xtra"/><references/>
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*[[Green Fluorescent Protein|Green Fluorescent Protein]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
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[[Category: Watkins, J L.]]
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[[Category: Watkins, J L]]
[[Category: Fluorescent protein]]
[[Category: Fluorescent protein]]
[[Category: Gfp-like beta-barrel]]
[[Category: Gfp-like beta-barrel]]

Revision as of 11:45, 21 December 2014

The 1.62A structure of a FRET-optimized Cerulean Fluorescent Protein

4en1, resolution 1.62Å

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