4hti
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==Crystallographic structure of the membrane-proximal ectodomain of the human receptor-type protein-tyrosine phosphatase phogrin== | |
| - | + | <StructureSection load='4hti' size='340' side='right' caption='[[4hti]], [[Resolution|resolution]] 1.95Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[4hti]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HTI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HTI FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PTPRN2, KIAA0387 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> |
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hti OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hti RCSB], [http://www.ebi.ac.uk/pdbsum/4hti PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/PTPR2_HUMAN PTPR2_HUMAN]] Implicated in development of nervous system and pancreatic endocrine cells.<ref>PMID:8878534</ref> <ref>PMID:8798755</ref> | [[http://www.uniprot.org/uniprot/PTPR2_HUMAN PTPR2_HUMAN]] Implicated in development of nervous system and pancreatic endocrine cells.<ref>PMID:8878534</ref> <ref>PMID:8798755</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The receptor-type protein-tyrosine phosphatase (RPTP) phogrin is localized at the membrane of secretory granules of pancreatic islet beta-cells and, similarly to the closely related ICA512, plays a role in the regulation of insulin secretion, in ensuring proper granulogenesis and stability, and in the regulation of beta-cell growth. The mature membrane-proximal ectodomain of phogrin (MPE phogrin) was produced as a recombinant protein and characterized. CD, fluorescence, controlled proteolysis, size-exclusion chromatography, and multi-angle ligth scattering showed that it is a properly-folded monomeric domain. Equilibrium experiments, in the presence of guanidinium chloride and thermal unfolding, suggest a two-state mechanism with a DeltaG of 2.3-3.3 kcal/mol, respectively. The study establishes common features and differences of MPE phogrin and the homologous ectodomain of ICA512. A homology model of phogrin was built based in the x-ray structure of MPE ICA512. The model is a starting point for modeling the entire receptor and for testing the quaternary structure and interactions of this protein in vivo. A description of the membrane insertion mode and putative interacting surfaces of this large protein is fundamental for the understanding of its biological function. | ||
| + | |||
| + | Biophysical Characterization of the Membrane-proximal Ectodomain of the Receptor-type Protein-tyrosine Phosphatase Phogrin.,Noguera ME, Primo ME, Sosa LN, Risso VA, Poskus E, Ermacora MR Protein Pept Lett. 2012 Sep 25. PMID:23016632<ref>PMID:23016632</ref> | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | == | + | ==See Also== |
| - | + | *[[Antibody|Antibody]] | |
| + | *[[Tyrosine phosphatase|Tyrosine phosphatase]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein-tyrosine-phosphatase]] | [[Category: Protein-tyrosine-phosphatase]] | ||
| - | [[Category: Ermacora, M R | + | [[Category: Ermacora, M R]] |
| - | [[Category: Jakoncic, J | + | [[Category: Jakoncic, J]] |
| - | [[Category: Noguera, M E | + | [[Category: Noguera, M E]] |
| - | [[Category: Poskus, E | + | [[Category: Poskus, E]] |
[[Category: Autoimmunity]] | [[Category: Autoimmunity]] | ||
[[Category: Diabetes]] | [[Category: Diabetes]] | ||
Revision as of 18:29, 24 December 2014
Crystallographic structure of the membrane-proximal ectodomain of the human receptor-type protein-tyrosine phosphatase phogrin
| |||||||||||
