2iu5
From Proteopedia
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| - | [[Image:2iu5.gif|left|200px]] | + | [[Image:2iu5.gif|left|200px]] |
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| - | '''DIHYDROXYACETONE KINASE OPERON ACTIVATOR DHAS''' | + | {{Structure |
| + | |PDB= 2iu5 |SIZE=350|CAPTION= <scene name='initialview01'>2iu5</scene>, resolution 1.60Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''DIHYDROXYACETONE KINASE OPERON ACTIVATOR DHAS''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2IU5 is a [ | + | 2IU5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis_il1403 Lactococcus lactis subsp. lactis il1403]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IU5 OCA]. |
==Reference== | ==Reference== | ||
| - | Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators., Christen S, Srinivas A, Bahler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B, J Biol Chem. 2006 Aug 11;281(32):23129-37. Epub 2006 Jun 7. PMID:[http:// | + | Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators., Christen S, Srinivas A, Bahler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B, J Biol Chem. 2006 Aug 11;281(32):23129-37. Epub 2006 Jun 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16760471 16760471] |
[[Category: Lactococcus lactis subsp. lactis il1403]] | [[Category: Lactococcus lactis subsp. lactis il1403]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: activator]] | [[Category: activator]] | ||
[[Category: dihydroxyacetone]] | [[Category: dihydroxyacetone]] | ||
| - | [[Category: lactococcus | + | [[Category: lactococcus lacti]] |
[[Category: synthase]] | [[Category: synthase]] | ||
[[Category: tetr family]] | [[Category: tetr family]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:33:23 2008'' |
Revision as of 15:33, 20 March 2008
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| , resolution 1.60Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
DIHYDROXYACETONE KINASE OPERON ACTIVATOR DHAS
Overview
Dihydroxyacetone (Dha) kinases are a novel family of kinases with signaling and metabolic functions. Here we report the x-ray structures of the transcriptional activator DhaS and the coactivator DhaQ and characterize their function. DhaQ is a paralog of the Dha binding Dha kinase subunit; DhaS belongs to the family of TetR repressors although, unlike all known members of this family, it is a transcriptional activator. DhaQ and DhaS form a stable complex that in the presence of Dha activates transcription of the Lactococcus lactis dha operon. Dha covalently binds to DhaQ through a hemiaminal bond with a histidine and thereby induces a conformational change, which is propagated to the surface via a cantilever-like structure. DhaS binding protects an inverted repeat whose sequence is GGACACATN6ATTTGTCC and renders two GC base pairs of the operator DNA hypersensitive to DNase I cleavage. The proximal half-site of the inverted repeat partially overlaps with the predicted -35 consensus sequence of the dha promoter.
About this Structure
2IU5 is a Single protein structure of sequence from Lactococcus lactis subsp. lactis il1403. Full crystallographic information is available from OCA.
Reference
Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators., Christen S, Srinivas A, Bahler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B, J Biol Chem. 2006 Aug 11;281(32):23129-37. Epub 2006 Jun 7. PMID:16760471
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