4lf0

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'''Unreleased structure'''
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{{STRUCTURE_4lf0| PDB=4lf0 | SCENE= }}
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===The E142D mutant of the amidase from Geobacillus pallidus===
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The entry 4lf0 is ON HOLD until Paper Publication
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==Function==
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[[http://www.uniprot.org/uniprot/AMIE_BACSP AMIE_BACSP]] Catalyzes the hydrolysis of short-chain aliphatic amides to their corresponding organic acids with release of ammonia.<ref>PMID:10978771</ref> Also exhibits in vitro acyl transferase activity, transferring the acyl moiety of short-chain amides to hydroxylamine to form hydroxamates (By similarity).<ref>PMID:10978771</ref>
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Authors: Sewell, B.T., Weber, B.W., Kimani, S.W., Cowan, D.A., Hunter, R., Venter, G.A., Gumbart, J.C., Thuku, R.N., Varsani, A.
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==About this Structure==
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[[4lf0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp. Bacillus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LF0 OCA].
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Description: The E142D mutant of the amidase from Geobacillus pallidus
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==Reference==
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<references group="xtra"/><references/>
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[[Category: Amidase]]
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[[Category: Bacillus sp.]]
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[[Category: Cowan, D A.]]
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[[Category: Gumbart, J C.]]
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[[Category: Hunter, R.]]
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[[Category: Kimani, S W.]]
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[[Category: Sewell, B T.]]
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[[Category: Thuku, R N.]]
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[[Category: Varsani, A.]]
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[[Category: Venter, G A.]]
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[[Category: Weber, B W.]]
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[[Category: Alpha-beta-beta-alpha sandwich]]
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[[Category: Hydrolase]]

Revision as of 08:37, 21 August 2013

Template:STRUCTURE 4lf0

Contents

The E142D mutant of the amidase from Geobacillus pallidus

Function

[AMIE_BACSP] Catalyzes the hydrolysis of short-chain aliphatic amides to their corresponding organic acids with release of ammonia.[1] Also exhibits in vitro acyl transferase activity, transferring the acyl moiety of short-chain amides to hydroxylamine to form hydroxamates (By similarity).[2]

About this Structure

4lf0 is a 1 chain structure with sequence from Bacillus sp.. Full crystallographic information is available from OCA.

Reference

  1. Kim S, Oriel P. Cloning and expression of the nitrile hydratase and amidase genes from Bacillus sp. BR449 into Escherichia coli. Enzyme Microb Technol. 2000 Oct 1;27(7):492-501. PMID:10978771
  2. Kim S, Oriel P. Cloning and expression of the nitrile hydratase and amidase genes from Bacillus sp. BR449 into Escherichia coli. Enzyme Microb Technol. 2000 Oct 1;27(7):492-501. PMID:10978771

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