2j41
From Proteopedia
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| - | [[Image:2j41.jpg|left|200px]] | + | [[Image:2j41.jpg|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF STAPHYLOCOCCUS AUREUS GUANYLATE MONOPHOSPHATE KINASE''' | + | {{Structure |
| + | |PDB= 2j41 |SIZE=350|CAPTION= <scene name='initialview01'>2j41</scene>, resolution 1.90Å | ||
| + | |SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+C+1198'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+B+1206'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+D+1207'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+A+1198'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+D+1208'>AC5</scene>, <scene name='pdbsite=AC6:K+Binding+Site+For+Residue+B+1207'>AC6</scene>, <scene name='pdbsite=AC7:K+Binding+Site+For+Residue+C+1199'>AC7</scene>, <scene name='pdbsite=AC8:K+Binding+Site+For+Residue+D+1209'>AC8</scene>, <scene name='pdbsite=AC9:5gp+Binding+Site+For+Residue+C+1200'>AC9</scene>, <scene name='pdbsite=BC1:5gp+Binding+Site+For+Residue+D+1210'>BC1</scene> and <scene name='pdbsite=BC2:5gp+Binding+Site+For+Residue+B+1208'>BC2</scene> | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene> and <scene name='pdbligand=5GP:GUANOSINE-5'-MONOPHOSPHATE'>5GP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Guanylate_kinase Guanylate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.8 2.7.4.8] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF STAPHYLOCOCCUS AUREUS GUANYLATE MONOPHOSPHATE KINASE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2J41 is a [ | + | 2J41 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J41 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of Staphylococcus aureus guanylate monophosphate kinase., El Omari K, Dhaliwal B, Lockyer M, Charles I, Hawkins AR, Stammers DK, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Oct 1;62(Pt, 10):949-53. Epub 2006 Sep 19. PMID:[http:// | + | Structure of Staphylococcus aureus guanylate monophosphate kinase., El Omari K, Dhaliwal B, Lockyer M, Charles I, Hawkins AR, Stammers DK, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Oct 1;62(Pt, 10):949-53. Epub 2006 Sep 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17012781 17012781] |
[[Category: Guanylate kinase]] | [[Category: Guanylate kinase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:36:55 2008'' |
Revision as of 15:36, 20 March 2008
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| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , , , , , , , and | ||||||
| Ligands: | , and | ||||||
| Activity: | Guanylate kinase, with EC number 2.7.4.8 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF STAPHYLOCOCCUS AUREUS GUANYLATE MONOPHOSPHATE KINASE
Overview
Nucleotide monophosphate kinases (NMPKs) are potential antimicrobial drug targets owing to their role in supplying DNA and RNA precursors. The present work reports the crystal structure of Staphylococcus aureus guanylate monophosphate kinase (SaGMK) at 1.9 A resolution. The structure shows that unlike most GMKs SaGMK is dimeric, confirming the role of the extended C-terminus in dimer formation as first observed for Escherichia coli GMK (EcGMK). One of the two SaGMK dimers within the crystal asymmetric unit has two monomers in different conformations: an open form with a bound sulfate ion (mimicking the beta-phosphate of ATP) and a closed form with bound GMP and sulfate ion. GMP-induced domain movements in SaGMK can thus be defined by comparison of these conformational states. Like other GMKs, the binding of GMP firstly triggers a partial closure of the enzyme, diminishing the distance between the GMP-binding and ATP-binding sites. In addition, the closed structure shows the presence of a potassium ion in contact with the guanine ring of GMP. The potassium ion appears to form an integral part of the GMP-binding site, as the Tyr36 side chain has significantly moved to form a metal ion-ligand coordination involving the lone pair of the side-chain O atom. The potassium-binding site might also be exploited in the design of novel inhibitors.
About this Structure
2J41 is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
Reference
Structure of Staphylococcus aureus guanylate monophosphate kinase., El Omari K, Dhaliwal B, Lockyer M, Charles I, Hawkins AR, Stammers DK, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Oct 1;62(Pt, 10):949-53. Epub 2006 Sep 19. PMID:17012781
Page seeded by OCA on Thu Mar 20 17:36:55 2008
Categories: Guanylate kinase | Single protein | Staphylococcus aureus | Charles, I. | Dhaliwal, B. | Hawkins, A R. | Lockyer, M. | Omari, K El. | Stammers, D K. | 5GP | K | SO4 | Atp-binding | Gmk | Gmp | Kinase | Nucleotide-binding | Transferase
