4a82
From Proteopedia
(Difference between revisions)
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| - | + | ==Fitted model of staphylococcus aureus sav1866 model ABC transporter in the human cystic fibrosis transmembrane conductance regulator volume map EMD-1966.== | |
| - | + | <StructureSection load='4a82' size='340' side='right' caption='[[4a82]], [[Resolution|resolution]] 2.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4a82]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A82 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A82 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a82 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a82 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a82 RCSB], [http://www.ebi.ac.uk/pdbsum/4a82 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cystic fibrosis affects about 1 in 2500 live births and involves loss of transmembrane chloride flux due to a lack of a membrane protein channel termed the cystic fibrosis transmembrane conductance regulator (CFTR). We have studied CFTR structure by electron crystallography. The data were compared with existing structures of other ATP-binding cassette transporters. The protein was crystallized in the outward facing state and resembled the well characterized Sav1866 transporter. We identified regions in the CFTR map, not accounted for by Sav1866, which were potential locations for the regulatory region as well as the channel gate. In this analysis, we were aided by the fact that the unit cell was composed of two molecules not related by crystallographic symmetry. We also identified regions in the fitted Sav1866 model that were missing from the map, hence regions that were either disordered in CFTR or differently organized compared with Sav1866. Apart from the N and C termini, this indicated that in CFTR, the cytoplasmic end of transmembrane helix 5/11 and its associated loop could be partly disordered (or alternatively located). | ||
| - | + | The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR): THREE-DIMENSIONAL STRUCTURE AND LOCALIZATION OF A CHANNEL GATE.,Rosenberg MF, O'Ryan LP, Hughes G, Zhao Z, Aleksandrov LA, Riordan JR, Ford RC J Biol Chem. 2011 Dec 9;286(49):42647-54. Epub 2011 Sep 19. PMID:21931164<ref>PMID:21931164</ref> | |
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| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Aleksandrov, L A | + | [[Category: Aleksandrov, L A]] |
| - | [[Category: Ford, R C | + | [[Category: Ford, R C]] |
| - | [[Category: Hughes, G | + | [[Category: Hughes, G]] |
| - | [[Category: ORyan, L P | + | [[Category: ORyan, L P]] |
| - | [[Category: Riordan, J R | + | [[Category: Riordan, J R]] |
| - | [[Category: Rosenberg, M F | + | [[Category: Rosenberg, M F]] |
| - | [[Category: Zhao, Z | + | [[Category: Zhao, Z]] |
[[Category: Cassette protein]] | [[Category: Cassette protein]] | ||
[[Category: Cftr]] | [[Category: Cftr]] | ||
Revision as of 09:37, 21 December 2014
Fitted model of staphylococcus aureus sav1866 model ABC transporter in the human cystic fibrosis transmembrane conductance regulator volume map EMD-1966.
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