2jeu
From Proteopedia
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- | [[Image:2jeu.jpg|left|200px]] | + | [[Image:2jeu.jpg|left|200px]] |
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- | '''TRANSCRIPTION ACTIVATOR STRUCTURE REVEALS REDOX CONTROL OF A REPLICATION INITIATION REACTION''' | + | {{Structure |
+ | |PDB= 2jeu |SIZE=350|CAPTION= <scene name='initialview01'>2jeu</scene>, resolution 2.80Å | ||
+ | |SITE= | ||
+ | |LIGAND= | ||
+ | |ACTIVITY= | ||
+ | |GENE= | ||
+ | }} | ||
+ | |||
+ | '''TRANSCRIPTION ACTIVATOR STRUCTURE REVEALS REDOX CONTROL OF A REPLICATION INITIATION REACTION''' | ||
+ | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
- | 2JEU is a [ | + | 2JEU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bovine_papillomavirus_type_1 Bovine papillomavirus type 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JEU OCA]. |
==Reference== | ==Reference== | ||
- | Transcription activator structure reveals redox control of a replication initiation reaction., Sanders CM, Sizov D, Seavers PR, Ortiz-Lombardia M, Antson AA, Nucleic Acids Res. 2007;35(10):3504-15. Epub 2007 May 3. PMID:[http:// | + | Transcription activator structure reveals redox control of a replication initiation reaction., Sanders CM, Sizov D, Seavers PR, Ortiz-Lombardia M, Antson AA, Nucleic Acids Res. 2007;35(10):3504-15. Epub 2007 May 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17478495 17478495] |
[[Category: Bovine papillomavirus type 1]] | [[Category: Bovine papillomavirus type 1]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: viral transcription factor]] | [[Category: viral transcription factor]] | ||
- | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:41:00 2008'' |
Revision as of 15:41, 20 March 2008
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, resolution 2.80Å | |||||||
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Coordinates: | save as pdb, mmCIF, xml |
TRANSCRIPTION ACTIVATOR STRUCTURE REVEALS REDOX CONTROL OF A REPLICATION INITIATION REACTION
Overview
Redox changes are one of the factors that influence cell-cycle progression and that control the processes of cellular proliferation, differentiation, senescence and apoptosis. Proteins regulated through redox-sensitive cysteines have been characterized but specific 'sulphydryl switches' in replication proteins remain to be identified. In bovine papillomavirus type-1, DNA replication begins when the viral transcription factor E2 recruits the viral initiator protein E1 to the origin of DNA replication (ori). Here we show that a novel dimerization interface in the E2 transcription activation domain is stabilized by a disulphide bond. Oxidative cross-linking via Cys57 sequesters the interaction surface between E1 and E2, preventing pre-initiation and replication initiation complex formation. Our data demonstrate that as well as a mechanism for regulating DNA binding, redox reactions can control replication by modulating the tertiary structure of critical protein factors using a specific redox sensor.
About this Structure
2JEU is a Single protein structure of sequence from Bovine papillomavirus type 1. Full crystallographic information is available from OCA.
Reference
Transcription activator structure reveals redox control of a replication initiation reaction., Sanders CM, Sizov D, Seavers PR, Ortiz-Lombardia M, Antson AA, Nucleic Acids Res. 2007;35(10):3504-15. Epub 2007 May 3. PMID:17478495
Page seeded by OCA on Thu Mar 20 17:41:00 2008
Categories: Bovine papillomavirus type 1 | Single protein | Antson, A A. | Ortiz-Lombardia, M. | Sanders, C M. | Seavers, P R. | Sizov, D. | Activator | Bovine papillomavirus | Dna replication | Dna-binding | E1 | E2 | Early protein | Nuclear protein | Oxidation | Phosphorylation | Redox control | Replication initiation | Repressor | Transcription | Transcription regulation | Viral transcription factor