2jet
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2jet.jpg|left|200px]] | + | [[Image:2jet.jpg|left|200px]] |
| - | + | ||
| - | '''CRYSTAL STRUCTURE OF A TRYPSIN-LIKE MUTANT (S189D, A226G) CHYMOTRYPSIN.''' | + | {{Structure |
| + | |PDB= 2jet |SIZE=350|CAPTION= <scene name='initialview01'>2jet</scene>, resolution 2.20Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF A TRYPSIN-LIKE MUTANT (S189D, A226G) CHYMOTRYPSIN.''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2JET is a [ | + | 2JET is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JET OCA]. |
==Reference== | ==Reference== | ||
| - | The Crystal Structure of a Trypsin-like Mutant Chymotrypsin: The Role of Position 226 in the Activity and Specificity of S189D Chymotrypsin., Jelinek B, Katona G, Fodor K, Venekei I, Graf L, Protein J. 2007 Sep 6;. PMID:[http:// | + | The Crystal Structure of a Trypsin-like Mutant Chymotrypsin: The Role of Position 226 in the Activity and Specificity of S189D Chymotrypsin., Jelinek B, Katona G, Fodor K, Venekei I, Graf L, Protein J. 2007 Sep 6;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17805946 17805946] |
[[Category: Chymotrypsin]] | [[Category: Chymotrypsin]] | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
| Line 27: | Line 36: | ||
[[Category: zymogen]] | [[Category: zymogen]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:40:58 2008'' |
Revision as of 15:40, 20 March 2008
| |||||||
| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Chymotrypsin, with EC number 3.4.21.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF A TRYPSIN-LIKE MUTANT (S189D, A226G) CHYMOTRYPSIN.
Overview
The crystal structure of the S189D+A226G rat chymotrypsin-B mutant has been determined at 2.2 A resolution. This mutant is the most trypsin-like mutant so far in the line of chymotrypsin-to-trypsin conversions, aiming for a more complete understanding of the structural basis of substrate specificity in pancreatic serine proteases. A226G caused significant rearrangements relative to S189D chymotrypsin, allowing an internal conformation of Asp189 which is close to that in trypsin. Serious distortions remain, however, in the activation domain, including zymogen-like features. The pH-profile of activity suggests that the conformation of the S1-site of the mutant is influenced also by the P1 residue of the substrate.
About this Structure
2JET is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
The Crystal Structure of a Trypsin-like Mutant Chymotrypsin: The Role of Position 226 in the Activity and Specificity of S189D Chymotrypsin., Jelinek B, Katona G, Fodor K, Venekei I, Graf L, Protein J. 2007 Sep 6;. PMID:17805946
Page seeded by OCA on Thu Mar 20 17:40:58 2008
