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2jgp

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[[Image:2jgp.gif|left|200px]]<br /><applet load="2jgp" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2jgp.gif|left|200px]]
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caption="2jgp, resolution 1.85&Aring;" />
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'''STRUCTURE OF THE TYCC5-6 PCP-C BIDOMAIN OF THE TYROCIDINE SYNTHETASE TYCC'''<br />
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{{Structure
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|PDB= 2jgp |SIZE=350|CAPTION= <scene name='initialview01'>2jgp</scene>, resolution 1.85&Aring;
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|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Dio+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Dio+Binding+Site+For+Chain+A'>AC4</scene> and <scene name='pdbsite=AC5:Na+Binding+Site+For+Chain+A'>AC5</scene>
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=DIO:1,4-DIETHYLENE DIOXIDE'>DIO</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''STRUCTURE OF THE TYCC5-6 PCP-C BIDOMAIN OF THE TYROCIDINE SYNTHETASE TYCC'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2JGP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Brevibacillus_brevis Brevibacillus brevis] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=DIO:'>DIO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Dio+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Dio+Binding+Site+For+Chain+A'>AC4</scene> and <scene name='pdbsite=AC5:Na+Binding+Site+For+Chain+A'>AC5</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JGP OCA].
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2JGP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Brevibacillus_brevis Brevibacillus brevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JGP OCA].
==Reference==
==Reference==
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Structural and functional insights into a peptide bond-forming bidomain from a nonribosomal peptide synthetase., Samel SA, Schoenafinger G, Knappe TA, Marahiel MA, Essen LO, Structure. 2007 Jul;15(7):781-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17637339 17637339]
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Structural and functional insights into a peptide bond-forming bidomain from a nonribosomal peptide synthetase., Samel SA, Schoenafinger G, Knappe TA, Marahiel MA, Essen LO, Structure. 2007 Jul;15(7):781-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17637339 17637339]
[[Category: Brevibacillus brevis]]
[[Category: Brevibacillus brevis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: SO4]]
[[Category: SO4]]
[[Category: antibiotic biosynthesis]]
[[Category: antibiotic biosynthesis]]
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[[Category: antibiotics]]
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[[Category: antibiotic]]
[[Category: condensation domain]]
[[Category: condensation domain]]
[[Category: ligase]]
[[Category: ligase]]
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[[Category: tyrocidine]]
[[Category: tyrocidine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:03:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:41:40 2008''

Revision as of 15:41, 20 March 2008

Image:2jgp.gif


PDB ID 2jgp

Drag the structure with the mouse to rotate
, resolution 1.85Å
Sites: , , , and
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF THE TYCC5-6 PCP-C BIDOMAIN OF THE TYROCIDINE SYNTHETASE TYCC


Overview

The crystal structure of the bidomain PCP-C from modules 5 and 6 of the nonribosomal tyrocidine synthetase TycC was determined at 1.8 A resolution. The bidomain structure reveals a V-shaped condensation domain, the canyon-like active site groove of which is associated with the preceding peptidyl carrier protein (PCP) domain at its donor side. The relative arrangement of the PCP and the peptide bond-forming condensation (C) domain places the active sites approximately 50 A apart. Accordingly, this PCP-C structure represents a conformational state prior to peptide transfer from the donor-PCP to the acceptor-PCP domain, implying the existence of additional states of PCP-C domain interaction during catalysis. Additionally, PCP-C exerts a mode of cyclization activity that mimics peptide bond formation catalyzed by C domains. Based on mutational data and pK value analysis of active site residues, it is suggested that nonribosomal peptide bond formation depends on electrostatic interactions rather than on general acid/base catalysis.

About this Structure

2JGP is a Single protein structure of sequence from Brevibacillus brevis. Full crystallographic information is available from OCA.

Reference

Structural and functional insights into a peptide bond-forming bidomain from a nonribosomal peptide synthetase., Samel SA, Schoenafinger G, Knappe TA, Marahiel MA, Essen LO, Structure. 2007 Jul;15(7):781-92. PMID:17637339

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