4bx9
From Proteopedia
(Difference between revisions)
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| - | + | ==Human Vps33A in complex with a fragment of human Vps16== | |
| - | + | <StructureSection load='4bx9' size='340' side='right' caption='[[4bx9]], [[Resolution|resolution]] 2.60Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4bx9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BX9 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=LMR:(2S)-2-HYDROXYBUTANEDIOIC+ACID'>LMR</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bx8|4bx8]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bx9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bx9 RCSB], [http://www.ebi.ac.uk/pdbsum/4bx9 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The multisubunit homotypic fusion and vacuole protein sorting (HOPS) membrane-tethering complex is required for late endosome-lysosome and autophagosome-lysosome fusion in mammals. We have determined the crystal structure of the human HOPS subunit Vps33A, confirming its identity as a Sec1/Munc18 family member. We show that HOPS subunit Vps16 recruits Vps33A to the human HOPS complex and that residues 642-736 are necessary and sufficient for this interaction, and we present the crystal structure of Vps33A in complex with Vps16(642-736). Mutations at the binding interface disrupt the Vps33A-Vps16 interaction both in vitro and in cells, preventing recruitment of Vps33A to the HOPS complex. The Vps33A-Vps16 complex provides a structural framework for studying the association between Sec1/Munc18 proteins and tethering complexes. | ||
| - | + | Structural basis of Vps33A recruitment to the human HOPS complex by Vps16.,Graham SC, Wartosch L, Gray SR, Scourfield EJ, Deane JE, Luzio JP, Owen DJ Proc Natl Acad Sci U S A. 2013 Jul 30. PMID:23901104<ref>PMID:23901104</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Deane, J E | + | [[Category: Deane, J E]] |
| - | [[Category: Graham, S C | + | [[Category: Graham, S C]] |
| - | [[Category: Gray, S R | + | [[Category: Gray, S R]] |
| - | [[Category: Luzio, J P | + | [[Category: Luzio, J P]] |
| - | [[Category: Owen, D J | + | [[Category: Owen, D J]] |
| - | [[Category: Scourfield, E J | + | [[Category: Scourfield, E J]] |
| - | [[Category: Wartosch, L | + | [[Category: Wartosch, L]] |
[[Category: Hop]] | [[Category: Hop]] | ||
[[Category: Membrane trafficking]] | [[Category: Membrane trafficking]] | ||
Revision as of 08:57, 21 December 2014
Human Vps33A in complex with a fragment of human Vps16
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