4bf7
From Proteopedia
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| - | + | ==Emericilla nidulans endo-beta-1,4-galactanase== | |
| - | === | + | <StructureSection load='4bf7' size='340' side='right' caption='[[4bf7]], [[Resolution|resolution]] 2.00Å' scene=''> |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[4bf7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BF7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BF7 FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinogalactan_endo-beta-1,4-galactanase Arabinogalactan endo-beta-1,4-galactanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.89 3.2.1.89] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bf7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bf7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bf7 RCSB], [http://www.ebi.ac.uk/pdbsum/4bf7 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/GANA_EMENI GANA_EMENI]] Endogalactanase involved in the degradation of plant cell wall polysaccharides, and more particularly of hairy regions of pectin. | [[http://www.uniprot.org/uniprot/GANA_EMENI GANA_EMENI]] Endogalactanase involved in the degradation of plant cell wall polysaccharides, and more particularly of hairy regions of pectin. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A novel Emericella nidulans endo-beta-1,4-galactanase (EnGAL) demonstrates a strong capacity to generate high levels of very potent prebiotic oligosaccharides from potato pulp, a by-product of the agricultural potato-starch industry. EnGAL belongs to glycoside hydrolase family 53 and shows high (72.5%) sequence identity to an endo-beta-1,4-galactanase from Aspergillus aculeatus. Diffraction data extending to 2.0 A resolution were collected from a crystal of EnGAL grown from conditions containing 0.2 M zinc acetate. The crystal structure showed a high similarity between EnGAL and other endo-beta-1,4-galactanases belonging to GH53. It also revealed 15 zinc ions bound to the protein, one of which is located in the active site, where it is coordinated by residues Glu136 and Glu246 which comprise the catalytic machinery. The majority of the zinc ions are located on the surface of the enzyme, in some cases with side chains from two different molecules as ligands, thus explaining why the presence of zinc ions was essential for crystallization. | ||
| - | + | The binding of zinc ions to Emericella nidulans endo-beta-1,4-galactanase is essential for crystal formation.,Otten H, Michalak M, Mikkelsen JD, Larsen S Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Aug;69(Pt 8):850-4. doi:, 10.1107/S1744309113019714. Epub 2013 Jul 27. PMID:23908026<ref>PMID:23908026</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Arabinogalactan endo-beta-1,4-galactanase]] | [[Category: Arabinogalactan endo-beta-1,4-galactanase]] | ||
[[Category: Emericella nidulans]] | [[Category: Emericella nidulans]] | ||
| - | [[Category: Larsen, S | + | [[Category: Larsen, S]] |
| - | [[Category: Michalak, M | + | [[Category: Michalak, M]] |
| - | [[Category: Mikkelsen, J D | + | [[Category: Mikkelsen, J D]] |
| - | [[Category: Otten, H | + | [[Category: Otten, H]] |
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
Revision as of 19:28, 25 December 2014
Emericilla nidulans endo-beta-1,4-galactanase
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