4jyc
From Proteopedia
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| - | + | ==MeaB, A Bacterial Homolog of MMAA, in its Apo form== | |
| - | + | <StructureSection load='4jyc' size='340' side='right' caption='[[4jyc]], [[Resolution|resolution]] 2.20Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4jyc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Methylobacterium_extorquens_am1 Methylobacterium extorquens am1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JYC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JYC FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2qm7|2qm7]], [[2qm8|2qm8]], [[4jyb|4jyb]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">meaB, MexAM1_META1p0188 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272630 Methylobacterium extorquens AM1])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jyc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jyc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jyc RCSB], [http://www.ebi.ac.uk/pdbsum/4jyc PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Fidelity during cofactor assembly is essential for the proper functioning of metalloenzymes and is ensured by specific chaperones. MeaB, a G-protein chaperone for the coenzyme B12-dependent radical enzyme methylmalonyl-CoA mutase (MCM), uses the energy of GTP binding, hydrolysis or both to regulate cofactor loading into MCM, protect MCM from inactivation and rescue MCM that is inactivated during turnover. Typically, G proteins signal to client proteins using the conformationally mobile switch I and II loops. Crystallographic snapshots of MeaB reported herein reveal a new switch III element that has substantial conformational plasticity. Using alanine-scanning mutagenesis, we demonstrate that the switch III motif is critical for bidirectional signal transmission of the GTPase-activating protein activity of MCM and the chaperone functions of MeaB in the MeaB-MCM complex. Mutations in the switch III loop identified in patients corrupt this interprotein communication and lead to methylmalonic aciduria, an inborn error of metabolism. | ||
| - | + | A switch III motif relays signaling between a B enzyme and its G-protein chaperone.,Lofgren M, Padovani D, Koutmos M, Banerjee R Nat Chem Biol. 2013 Jul 21. doi: 10.1038/nchembio.1298. PMID:23873214<ref>PMID:23873214</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Methylobacterium extorquens am1]] | [[Category: Methylobacterium extorquens am1]] | ||
| - | [[Category: Banerjee, R | + | [[Category: Banerjee, R]] |
| - | [[Category: Koutmos, M | + | [[Category: Koutmos, M]] |
| - | [[Category: Lofgren, M | + | [[Category: Lofgren, M]] |
| - | [[Category: Padovani, D | + | [[Category: Padovani, D]] |
[[Category: Alpha and beta protein]] | [[Category: Alpha and beta protein]] | ||
[[Category: Chaperone]] | [[Category: Chaperone]] | ||
Revision as of 10:08, 20 January 2015
MeaB, A Bacterial Homolog of MMAA, in its Apo form
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