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3w1e
From Proteopedia
(Difference between revisions)
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| - | + | ==Structure of FlgT, a flagellar basal body component protein== | |
| - | + | <StructureSection load='3w1e' size='340' side='right' caption='[[3w1e]], [[Resolution|resolution]] 2.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3w1e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_alginolyticus Vibrio alginolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W1E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W1E FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">flgT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=663 Vibrio alginolyticus])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w1e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3w1e RCSB], [http://www.ebi.ac.uk/pdbsum/3w1e PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Flagellar motility is a key factor for bacterial survival and growth in fluctuating environments. The polar flagellum of a marine bacterium, Vibrio alginolyticus, is driven by sodium ion influx and rotates approximately six times faster than the proton-driven motor of Escherichia coli. The basal body of the sodium motor has two unique ring structures, the T ring and the H ring. These structures are essential for proper assembly of the stator unit into the basal body and to stabilize the motor. FlgT, which is a flagellar protein specific for Vibrio sp., is required to form and stabilize both ring structures. Here, we report the crystal structure of FlgT at 2.0-A resolution. FlgT is composed of three domains, the N-terminal domain (FlgT-N), the middle domain (FlgT-M), and the C-terminal domain (FlgT-C). FlgT-M is similar to the N-terminal domain of TolB, and FlgT-C resembles the N-terminal domain of FliI and the alpha/beta subunits of F1-ATPase. To elucidate the role of each domain, we prepared domain deletion mutants of FlgT and analyzed their effects on the basal-body ring formation. The results suggest that FlgT-N contributes to the construction of the H-ring structure, and FlgT-M mediates the T-ring association on the LP ring. FlgT-C is not essential but stabilizes the H-ring structure. On the basis of these results, we propose an assembly mechanism for the basal-body rings and the stator units of the sodium-driven flagellar motor. | ||
| - | + | Insight into the assembly mechanism in the supramolecular rings of the sodium-driven Vibrio flagellar motor from the structure of FlgT.,Terashima H, Li N, Sakuma M, Koike M, Kojima S, Homma M, Imada K Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):6133-8. doi:, 10.1073/pnas.1222655110. Epub 2013 Mar 25. PMID:23530206<ref>PMID:23530206</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Vibrio alginolyticus]] | [[Category: Vibrio alginolyticus]] | ||
| - | [[Category: Homma, M | + | [[Category: Homma, M]] |
| - | [[Category: Imada, K | + | [[Category: Imada, K]] |
| - | [[Category: Sakuma, M | + | [[Category: Sakuma, M]] |
| - | [[Category: Terashima, H | + | [[Category: Terashima, H]] |
[[Category: H-ring formation]] | [[Category: H-ring formation]] | ||
[[Category: Motor protein]] | [[Category: Motor protein]] | ||
[[Category: The flagellar basal body]] | [[Category: The flagellar basal body]] | ||
Revision as of 10:26, 4 January 2015
Structure of FlgT, a flagellar basal body component protein
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