2jmp
From Proteopedia
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| - | [[Image:2jmp.jpg|left|200px]] | + | [[Image:2jmp.jpg|left|200px]] |
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| - | '''Structure for the N-terminus of chromosomal replication initiation protein dnaA from M. genitalium''' | + | {{Structure |
| + | |PDB= 2jmp |SIZE=350|CAPTION= <scene name='initialview01'>2jmp</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= dnaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2097 Mycoplasma genitalium]) | ||
| + | }} | ||
| + | |||
| + | '''Structure for the N-terminus of chromosomal replication initiation protein dnaA from M. genitalium''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2JMP is a [ | + | 2JMP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycoplasma_genitalium Mycoplasma genitalium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMP OCA]. |
==Reference== | ==Reference== | ||
| - | NMR structure of the N-terminal domain of the replication initiator protein DnaA., Lowery TJ, Pelton JG, Chandonia JM, Kim R, Yokota H, Wemmer DE, J Struct Funct Genomics. 2007 Mar;8(1):11-7. Epub 2007 Aug 7. PMID:[http:// | + | NMR structure of the N-terminal domain of the replication initiator protein DnaA., Lowery TJ, Pelton JG, Chandonia JM, Kim R, Yokota H, Wemmer DE, J Struct Funct Genomics. 2007 Mar;8(1):11-7. Epub 2007 Aug 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17680349 17680349] |
[[Category: Mycoplasma genitalium]] | [[Category: Mycoplasma genitalium]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
[[Category: psi]] | [[Category: psi]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:43:01 2008'' |
Revision as of 15:43, 20 March 2008
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| Gene: | dnaA (Mycoplasma genitalium) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure for the N-terminus of chromosomal replication initiation protein dnaA from M. genitalium
Overview
DnaA is an essential component in the initiation of bacterial chromosomal replication. DnaA binds to a series of 9 base pair repeats leading to oligomerization, recruitment of the DnaBC helicase, and the assembly of the replication fork machinery. The structure of the N-terminal domain (residues 1-100) of DnaA from Mycoplasma genitalium was determined by NMR spectroscopy. The backbone r.m.s.d. for the first 86 residues was 0.6 +/- 0.2 A based on 742 NOE, 50 hydrogen bond, 46 backbone angle, and 88 residual dipolar coupling restraints. Ultracentrifugation studies revealed that the domain is monomeric in solution. Features on the protein surface include a hydrophobic cleft flanked by several negative residues on one side, and positive residues on the other. A negatively charged ridge is present on the opposite face of the protein. These surfaces may be important sites of interaction with other proteins involved in the replication process. Together, the structure and NMR assignments should facilitate the design of new experiments to probe the protein-protein interactions essential for the initiation of DNA replication.
About this Structure
2JMP is a Single protein structure of sequence from Mycoplasma genitalium. Full crystallographic information is available from OCA.
Reference
NMR structure of the N-terminal domain of the replication initiator protein DnaA., Lowery TJ, Pelton JG, Chandonia JM, Kim R, Yokota H, Wemmer DE, J Struct Funct Genomics. 2007 Mar;8(1):11-7. Epub 2007 Aug 7. PMID:17680349
Page seeded by OCA on Thu Mar 20 17:43:01 2008
