2jni
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2jni.jpg|left|200px]] | + | [[Image:2jni.jpg|left|200px]] |
| - | + | ||
| - | '''Spatial structure of antimicrobial peptide arenicin-2 in aqueous solution''' | + | {{Structure |
| + | |PDB= 2jni |SIZE=350|CAPTION= <scene name='initialview01'>2jni</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Spatial structure of antimicrobial peptide arenicin-2 in aqueous solution''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2JNI is a [ | + | 2JNI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arenicola_marina Arenicola marina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNI OCA]. |
==Reference== | ==Reference== | ||
| - | Recombinant expression, synthesis, purification, and solution structure of arenicin., Ovchinnikova TV, Shenkarev ZO, Nadezhdin KD, Balandin SV, Zhmak MN, Kudelina IA, Finkina EI, Kokryakov VN, Arseniev AS, Biochem Biophys Res Commun. 2007 Aug 17;360(1):156-62. Epub 2007 Jun 14. PMID:[http:// | + | Recombinant expression, synthesis, purification, and solution structure of arenicin., Ovchinnikova TV, Shenkarev ZO, Nadezhdin KD, Balandin SV, Zhmak MN, Kudelina IA, Finkina EI, Kokryakov VN, Arseniev AS, Biochem Biophys Res Commun. 2007 Aug 17;360(1):156-62. Epub 2007 Jun 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17585874 17585874] |
[[Category: Arenicola marina]] | [[Category: Arenicola marina]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 27: | Line 36: | ||
[[Category: peptide]] | [[Category: peptide]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:43:13 2008'' |
Revision as of 15:43, 20 March 2008
| |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Spatial structure of antimicrobial peptide arenicin-2 in aqueous solution
Overview
Arenicins are 21-residue cationic antimicrobial peptides, isolated from marine polychaeta Arenicola marina. In order to determine a high-resolution three-dimensional structure of arenicin-2, the recombinant peptide was overexpressed as a fused form in Escherichia coli. Both arenicin isoforms were synthesized using the Fmoc-based solid-phase strategy. Recombinant and synthetic arenicins were purified, and their antimicrobial and spectroscopic properties were analyzed. NMR investigation shows that in water solution arenicin-2 displays a prolonged beta-hairpin, formed by two antiparallel beta-strands and stabilized by one disulfide and nine hydrogen bonds. A significant right-handed twist in the beta-sheet is deprived the peptide surface of amphipathicity. CD spectroscopic analysis indicates that arenicin-2 binds to the SDS and DPC micelles, and conformation of the peptide is significantly changed upon binding. Arenicin strongly binds to anionic lipid (POPE/POPG) vesicles in contrast with zwitterionic (POPC) ones. These results suggest that arenicins are membrane active peptides and point to possible mechanism of their selectivity toward bacterial cells.
About this Structure
2JNI is a Single protein structure of sequence from Arenicola marina. Full crystallographic information is available from OCA.
Reference
Recombinant expression, synthesis, purification, and solution structure of arenicin., Ovchinnikova TV, Shenkarev ZO, Nadezhdin KD, Balandin SV, Zhmak MN, Kudelina IA, Finkina EI, Kokryakov VN, Arseniev AS, Biochem Biophys Res Commun. 2007 Aug 17;360(1):156-62. Epub 2007 Jun 14. PMID:17585874
Page seeded by OCA on Thu Mar 20 17:43:13 2008
