2jou
From Proteopedia
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| - | [[Image:2jou.gif|left|200px]] | + | [[Image:2jou.gif|left|200px]] |
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| - | '''NMR structure of Mini-B, an N-terminal- C-terminal construct from human Surfactant Protein-B (SP-B), in Hexafluoroisopropanol (HFIP)''' | + | {{Structure |
| + | |PDB= 2jou |SIZE=350|CAPTION= <scene name='initialview01'>2jou</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''NMR structure of Mini-B, an N-terminal- C-terminal construct from human Surfactant Protein-B (SP-B), in Hexafluoroisopropanol (HFIP)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2JOU is a [ | + | 2JOU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. This structure supersedes the now removed PDB entry 2A2H. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JOU OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of mini-B, a functional fragment of surfactant protein B, in detergent micelles., Sarker M, Waring AJ, Walther FJ, Keough KM, Booth V, Biochemistry. 2007 Oct 2;46(39):11047-56. Epub 2007 Sep 11. PMID:[http:// | + | Structure of mini-B, a functional fragment of surfactant protein B, in detergent micelles., Sarker M, Waring AJ, Walther FJ, Keough KM, Booth V, Biochemistry. 2007 Oct 2;46(39):11047-56. Epub 2007 Sep 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17845058 17845058] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Booth, V.]] | [[Category: Booth, V.]] | ||
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[[Category: surfactant protein b]] | [[Category: surfactant protein b]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:43:41 2008'' |
Revision as of 15:43, 20 March 2008
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NMR structure of Mini-B, an N-terminal- C-terminal construct from human Surfactant Protein-B (SP-B), in Hexafluoroisopropanol (HFIP)
Contents |
Overview
Surfactant protein B (SP-B) is essential for normal lung surfactant function, which is in itself essential to life. However, the molecular basis for SP-B's activity is not understood and a high-resolution structure for SP-B has not been determined. Mini-B is a 34-residue peptide with internal disulfide linkages that is composed of the N- and C-terminal helical regions of SP-B. It has been shown to retain similar activity to full-length SP-B in certain in vitro and in vivo studies. We have used solution NMR to determine the structure of Mini-B in the presence of micelles composed of the anionic detergent sodium dodecyl sulfate (SDS). Under these conditions, Mini-B forms two alpha-helices connected by an unstructured loop. Mini-B possesses a strikingly amphipathic surface with a large positively charged patch on one face of the peptide and a large hydrophobic patch on the opposite face. A tryptophan side chain extends outward from the peptide in a position to interact with lipids at the polar/apolar interface. Interhelix interactions are stabilized by both disulfide bonds and by interleaving of hydrophobic side chains from the two helices.
Disease
Known disease associated with this structure: Surfactant metabolism dysfunction, pulmonary, 1 OMIM:[178640]
About this Structure
2JOU is a Single protein structure of sequence from [1]. This structure supersedes the now removed PDB entry 2A2H. Full crystallographic information is available from OCA.
Reference
Structure of mini-B, a functional fragment of surfactant protein B, in detergent micelles., Sarker M, Waring AJ, Walther FJ, Keough KM, Booth V, Biochemistry. 2007 Oct 2;46(39):11047-56. Epub 2007 Sep 11. PMID:17845058
Page seeded by OCA on Thu Mar 20 17:43:41 2008
