2jpd
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2jpd.jpg|left|200px]] | + | [[Image:2jpd.jpg|left|200px]] |
| - | + | ||
| - | '''Solution structure of the ERCC1 central domain''' | + | {{Structure |
| + | |PDB= 2jpd |SIZE=350|CAPTION= <scene name='initialview01'>2jpd</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= ERCC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Solution structure of the ERCC1 central domain''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 10: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2JPD is a [ | + | 2JPD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JPD OCA]. |
==Reference== | ==Reference== | ||
| - | Analysis of the XPA and ssDNA-binding surfaces on the central domain of human ERCC1 reveals evidence for subfunctionalization., Tripsianes K, Folkers GE, Zheng C, Das D, Grinstead JS, Kaptein R, Boelens R, Nucleic Acids Res. 2007;35(17):5789-98. Epub 2007 Aug 24. PMID:[http:// | + | Analysis of the XPA and ssDNA-binding surfaces on the central domain of human ERCC1 reveals evidence for subfunctionalization., Tripsianes K, Folkers GE, Zheng C, Das D, Grinstead JS, Kaptein R, Boelens R, Nucleic Acids Res. 2007;35(17):5789-98. Epub 2007 Aug 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17720715 17720715] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 26: | Line 35: | ||
[[Category: protein]] | [[Category: protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:43:55 2008'' |
Revision as of 15:43, 20 March 2008
| |||||||
| Gene: | ERCC1 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of the ERCC1 central domain
Contents |
Overview
Human ERCC1/XPF is a structure-specific endonuclease involved in multiple DNA repair pathways. We present the solution structure of the non-catalytic ERCC1 central domain. Although this domain shows structural homology with the catalytically active XPF nuclease domain, functional investigation reveals a completely distinct function for the ERCC1 central domain by performing interactions with both XPA and single-stranded DNA. These interactions are non-competitive and can occur simultaneously through distinct interaction surfaces. Interestingly, the XPA binding by ERCC1 and the catalytic function of XPF are dependent on a structurally homologous region of the two proteins. Although these regions are strictly conserved in each protein family, amino acid composition and surface characteristics are distinct. We discuss the possibility that after XPF gene duplication, the redundant ERCC1 central domain acquired novel functions, thereby increasing the fidelity of eukaryotic DNA repair.
Disease
Known disease associated with this structure: Cerebrooculofacioskeletal syndrome 4 OMIM:[126380]
About this Structure
2JPD is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Analysis of the XPA and ssDNA-binding surfaces on the central domain of human ERCC1 reveals evidence for subfunctionalization., Tripsianes K, Folkers GE, Zheng C, Das D, Grinstead JS, Kaptein R, Boelens R, Nucleic Acids Res. 2007;35(17):5789-98. Epub 2007 Aug 24. PMID:17720715
Page seeded by OCA on Thu Mar 20 17:43:55 2008
