1hax
From Proteopedia
(New page: 200px<br /> <applet load="1hax" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hax, resolution 1.6Å" /> '''SNAPSHOTS OF SERINE ...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1HAX is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with CA and SO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.11 3.4.21.11]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HAX OCA]]. | + | 1HAX is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with CA and SO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Hydrolase Hydrolase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.11 3.4.21.11]]. Structure known Active Site: CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HAX OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: serine proteinase]] | [[Category: serine proteinase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:41:51 2007'' |
Revision as of 06:37, 30 October 2007
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SNAPSHOTS OF SERINE PROTEASE CATALYSIS: (A) ACYL-ENZYME INTERMEDIATE BETWEEN PORCINE PANCREATIC ELASTASE AND HUMAN BETA-CASOMORPHIN-7 AT PH 5
Overview
Studies on the catalytic mechanism and inhibition of serine proteases are, widely used as paradigms for teaching enzyme catalysis. Ground-breaking, work on the structures of chymotrypsin and subtilisin led to the idea of a, conserved catalytic triad formed by the active site Ser, His and Asp, residues. An oxyanion hole, consisting of the peptide amide of the active, site serine and a neighbouring glycine, was identified, and hydrogen, bonding in the oxyanion hole was suggested to stabilize the two proposed, tetrahedral intermediates on the catalytic pathway. Here we show electron, density changes consistent with the formation of a tetrahedral, intermediate during the hydrolysis of an acyl-enzyme complex formed, between a natural heptapeptide and elastase. No electron density for an, ... [(full description)]
About this Structure
1HAX is a [Single protein] structure of sequence from [Homo sapiens] with CA and SO4 as [ligands]. Active as [Hydrolase], with EC number [3.4.21.11]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].
Reference
X-ray snapshots of serine protease catalysis reveal a tetrahedral intermediate., Wilmouth RC, Edman K, Neutze R, Wright PA, Clifton IJ, Schneider TR, Schofield CJ, Hajdu J, Nat Struct Biol. 2001 Aug;8(8):689-94. PMID:11473259
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