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2jub
From Proteopedia
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| - | [[Image:2jub.jpg|left|200px]] | + | [[Image:2jub.jpg|left|200px]] |
| - | + | ||
| - | '''Solution structure of IPI*''' | + | {{Structure |
| + | |PDB= 2jub |SIZE=350|CAPTION= <scene name='initialview01'>2jub</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= ipi1, ipi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12031 Enterobacteria phage TW28]) | ||
| + | }} | ||
| + | |||
| + | '''Solution structure of IPI*''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2JUB is a [ | + | 2JUB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_tw28 Enterobacteria phage tw28]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JUB OCA]. |
==Reference== | ==Reference== | ||
| - | Restriction endonuclease inhibitor IPI* of bacteriophage T4: a novel structure for a dedicated target., Rifat D, Wright NT, Varney KM, Weber DJ, Black LW, J Mol Biol. 2008 Jan 18;375(3):720-34. Epub 2007 Nov 1. PMID:[http:// | + | Restriction endonuclease inhibitor IPI* of bacteriophage T4: a novel structure for a dedicated target., Rifat D, Wright NT, Varney KM, Weber DJ, Black LW, J Mol Biol. 2008 Jan 18;375(3):720-34. Epub 2007 Nov 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18037438 18037438] |
[[Category: Enterobacteria phage tw28]] | [[Category: Enterobacteria phage tw28]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: t4 phage]] | [[Category: t4 phage]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:45:09 2008'' |
Revision as of 15:45, 20 March 2008
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| Gene: | ipi1, ipi (Enterobacteria phage TW28) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of IPI*
Overview
Phage T4 protects its DNA from the two-gene-encoded gmrS/gmrD (glucose-modified hydroxymethylcytosine restriction endonuclease) CT of pathogenic Escherichia coli, CT596, by injecting several hundred copies of the 76-amino-acid-residue nuclease inhibitor, IPI*, into the infected host. Here, the three-dimensional solution structure of mature IPI* is reported as determined by nuclear magnetic resonance techniques using 1290 experimental nuclear Overhauser effect and dipolar coupling constraints ( approximately 17 constraints per residue). Close examination of this oblate-shaped protein structure reveals a novel fold consisting of two small beta-sheets (beta1: B1 and B2; beta2: B3-B5) flanked at the N- and C-termini by alpha-helices (H1 and H2). Such a fold is very compact in shape and allows ejection of IPI* through the narrow 30-A portal and tail tube apertures of the virion without unfolding. Structural and dynamic measurements identify an exposed hydrophobic knob that is a putative gmrS/gmrD-binding site. A single gene from the uropathogenic E. coli UT189, which codes for a gmrS/gmrD-like UT fusion enzyme (with approximately 90% identity to the heterodimeric CT enzyme), has evolved IPI* inhibitor immunity. Analysis of the gmrS/gmrD restriction endonuclease enzyme family and its IPI* family phage antagonists reveals an evolutionary pathway that has elaborated a surprisingly diverse and specifically fitted set of coevolving attack and defense structures.
About this Structure
2JUB is a Single protein structure of sequence from Enterobacteria phage tw28. Full crystallographic information is available from OCA.
Reference
Restriction endonuclease inhibitor IPI* of bacteriophage T4: a novel structure for a dedicated target., Rifat D, Wright NT, Varney KM, Weber DJ, Black LW, J Mol Biol. 2008 Jan 18;375(3):720-34. Epub 2007 Nov 1. PMID:18037438
Page seeded by OCA on Thu Mar 20 17:45:09 2008
