4jeb

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{{STRUCTURE_4jeb| PDB=4jeb | SCENE= }}
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==Crystal structure of an engineered RIDC1 tetramer with four interfacial disulfide bonds and four three-coordinate Zn(II) sites==
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===Crystal structure of an engineered RIDC1 tetramer with four interfacial disulfide bonds and four three-coordinate Zn(II) sites===
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<StructureSection load='4jeb' size='340' side='right' caption='[[4jeb]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23905754}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4jeb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JEB FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hnk|3hnk]], [[2bc5|2bc5]], [[2qla|2qla]], [[3iq5|3iq5]], [[3iq6|3iq6]], [[3hni|3hni]], [[4je9|4je9]], [[4jea|4jea]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cybC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jeb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jeb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jeb RCSB], [http://www.ebi.ac.uk/pdbsum/4jeb PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Simultaneously strong and reversible through redox chemistry, disulfide bonds play a unique and often irreplaceable role in the formation of biological and synthetic assemblies. In an approach inspired by supramolecular chemistry, we report here that engineered noncovalent interactions on the surface of a monomeric protein can template its assembly into a unique cryptand-like protein complex ((C81/C96)RIDC14) by guiding the selective formation of multiple disulfide bonds across different interfaces. Owing to its highly interconnected framework, (C81/C96)RIDC14 is well preorganized for metal coordination in its interior, can support a large internal cavity surrounding the metal sites, and can withstand significant alterations in inner-sphere metal coordination. (C81/C96)RIDC14 self-assembles with high fidelity and yield in the periplasmic space of E. coli cells, where it can successfully compete for Zn(II) binding.
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==Function==
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In Vitro and Cellular Self-Assembly of a Zn-Binding Protein Cryptand via Templated Disulfide Bonds.,Medina-Morales A, Perez A, Brodin JD, Tezcan FA J Am Chem Soc. 2013 Aug 14;135(32):12013-22. doi: 10.1021/ja405318d. Epub 2013, Aug 1. PMID:23905754<ref>PMID:23905754</ref>
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[[http://www.uniprot.org/uniprot/C562_ECOLX C562_ECOLX]] Electron-transport protein of unknown function.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4jeb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JEB OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:023905754</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Brodin, J D.]]
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[[Category: Brodin, J D]]
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[[Category: Medina-Morales, A M.]]
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[[Category: Medina-Morales, A M]]
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[[Category: Perez, A.]]
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[[Category: Perez, A]]
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[[Category: Tezcan, F A.]]
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[[Category: Tezcan, F A]]
[[Category: Electron transport]]
[[Category: Electron transport]]
[[Category: Four-helix bundle]]
[[Category: Four-helix bundle]]

Revision as of 12:57, 21 December 2014

Crystal structure of an engineered RIDC1 tetramer with four interfacial disulfide bonds and four three-coordinate Zn(II) sites

4jeb, resolution 2.30Å

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